DNA encoding canine von Willebrand factor and methods of use
DCFirst Claim
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1. An isolated nucleic acid comprising a nucleotide sequence encoding the polypeptide of SEQ ID NO. 2, having a mutation at codon 85.
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Abstract
The complete sequence of the canine von Willebrand Factor cDNA and deduced amino acid sequence is provided. The mutation which causes von Willebrand'"'"'s Disease in Scottish Terriers, a single base deletion in exon 4, has also been determined. Methods for detecting carriers of the defective vWF gene are also provided.
7 Citations
15 Claims
- 1. An isolated nucleic acid comprising a nucleotide sequence encoding the polypeptide of SEQ ID NO. 2, having a mutation at codon 85.
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10. A method of detecting a canine von Willebrand Factor gene in a sample comprising the steps of:
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a) contacting the sample with an oligonucleotide comprising contiguous nucleotides of the nucleic acid sequence of SEQ ID NO. 1 having a base deletion at codon 85, and capable of specifically hybridizing with the canine von Willebrand Factor gene, under conditions favorable for hybridization of the oligonucleotide to any complementary sequences of nucleic acid in the sample; and
b) detecting hybridization, thereby detecting a canine von Willebrand Factor gene. - View Dependent Claims (11)
c) quantifying hybridization of the oligonucleotide to complementary sequence.
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12. A method of detecting a canine von Willebrand Factor gene in a sample comprising the steps of:
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a) contacting the sample with an oligonucleotide comprising contiguous nucleotides of the nucleic acid sequence that is complementary to the sequence of SEQ ID NO. 1 having a base deletion at codon 85, and capable of specifically hybridizing to the complementary nucleotide sequence, under conditions favorable for hybridization of the oligonucleotide to any complementary sequences of nucleic acid in the sample; and
b) detecting hybridization, thereby detecting a canine von Willebrand Factor gene. - View Dependent Claims (13)
c) quantifying ybidization of the oligonucleotide to complementary sequences.
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14. An assay kit for screening for a canine von Willebrand Factor gene comprising:
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a) an oligonucleotide comprising contiguous nucleotides from the nucleic acid sequence that is complementary to the sequence of SEQ ID NO. 1 having a base deletion at codon 85, and capable of specifically hybridizing to the complementary nucleotide sequence; and
b) reagents for hybridization of the oligonucleotide to a complementary nucleic acid sequence.
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15. An oligonucleotide probe capable of detecting a mutation associated with canine von Willebrand'"'"'s disease, wherein the mutation is a base deletion at codon 85 of the canine von Willebrand Factor gene.
Specification