Method and apparatus for cultivating a cell or tissue

• US 6,432,713 B2
• Filed: 03/02/2001
• Issued: 08/13/2002
• Est. Priority Date: 03/02/2000
• Status: Expired due to Term

• Current Assignee: Brigham and Women's Hospital Incorporated, Purpose Co., Ltd.
• Original Assignee: Takagi Industrial Company Limited
• Inventors: Watanabe, Setsuo, Takagi, Takao, Mizuno, Shuichi, Kinouchi, Ibuki, Glowacki, Julie, Takai, Hidetada
• Primary Examiner(s): Lankford, Jr., Leon B.
• Application Number:
  US09/796,422
• Publication Number:
  US 20010021529A1
• Time in Patent Office: 529 Days
• Field of Search: 435/383, 435/395
• US Class Current: 435/395
• CPC Class Codes: C12M 21/08 for producing artificial tissue or for ex-vivo cultivation of tissue prostheses A61F2/00, grafts A61L27/00
• CPC Class Codes: C12M 23/14 Bags
• CPC Class Codes: C12M 29/14 Pressurized fluid
• CPC Class Codes: C12M 35/04 Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
• CPC Class Codes: C12M 41/40 of pressure

Abstract

The invention provides a method of and an apparatus for cultivating a cell or tissue capable of preventing the cell or tissue from being contaminated and realizing an efficient in vitro culture. The method of and the apparatus for cultivating a cell or tissue comprise installing a culture position (culture chamber) under an environment that is arbitrarily controlled such as an environment mimicking the living body, supplying a culture medium to the cell or tissue while the cell or tissue is held at the culture position, and cultivating the cell or tissue at the culture position that is under the ideal environment, thereby preventing the cell or tissue from being contaminated and realizing an efficient in vitro culture.

Claims

1. A method of cultivating a cell or tissue of a living body to be cultivated inside a culture chamber comprising the steps of:

forming a sealed space while isolated from the outside into which space gas is supplied, and in which space temperature and humidity conditions are set to maintain an environment necessary for sustaining life;

forming a culture circuit unit which is separable from the sealed space;

the culture circuit unit comprising a culture fluid bath and the culture chamber which are installed therein and connected to each other by a tube to form a culture circuit through which circuit culture fluid can be circulated from the culture fluid bath to the culture chamber;

supplying the culture fluid continuously, intermittently or periodically or by the combination thereof from the culture fluid both so as to circulate the culture fluid in the culture chamber through the culture circuit;

rendering the cell or tissue in the culture chamber to be held in a suspending or non-suspending state by holding means in the culture fluid within the culture chamber; and

forming a gas absorption portion in the tube of the culture circuit at a front stage of the culture chamber separately from the culture fluid bath, through which the gas in the sealed space permeates and rendering the gas to be absorbed in the culture fluid which flows toward the culture chamber through the tube;

wherein when the cell or tissue held by the holding means grows, the holding means is absorbed in the cell or tissue and vanishes.

2. The method of cultivating a cell or tissue according to claim 1, wherein the cell or tissue is held in the culture fluid by a hydro-gel.

3. The method of cultivating a cell or tissue according to claim 1, wherein the culture fluid comprises at least one of the group consisting of amino acids, saccharides, salts and proteins.