Mystery swine disease diagnostic kits

• US 6,455,245 B1
• Filed: 05/05/2000
• Issued: 09/24/2002
• Est. Priority Date: 06/06/1991
• Status: Expired due to Fees

• Current Assignee: Stichting Dienst Landbouwkundig Onderzoek
• Original Assignee: Stichting Centraal Diergeneeskundig Instituut
• Inventors: Terpstra, Catharinus, Wensvoort, Gert, Meulenberg, Johanna Jacoba Maria, Moormann, Robertus Jacobus Maria, Pol, Joannes Maria Anthonis
• Primary Examiner(s): SALIMI, ALI REZA
• Application Number:
  US09/565,864
• Time in Patent Office: 872 Days
• Field of Search: 435/5, 435/7.9, 435/975, 435/7.94, 424/159.1, 424/211.1, 424/130.1
• US Class Current: 435/5
• CPC Class Codes: A61K 2039/525 Virus
• CPC Class Codes: A61K 2039/5252 inactivated (killed)
• CPC Class Codes: A61K 2039/5254 avirulent or attenuated
• CPC Class Codes: A61K 2039/53 DNA (RNA) vaccination
• CPC Class Codes: A61K 39/00 Medicinal preparations containing antigens or antibodies materials for immunoassay G01N33/53
• CPC Class Codes: A61K 39/12 Viral antigens
• CPC Class Codes: A61P 31/12 Antivirals
• CPC Class Codes: A61P 31/14 for RNA viruses
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C07K 16/10 from RNA viruses
• CPC Class Codes: C12N 2770/10021 Viruses as such, e.g. new isolates, mutants or their genomic sequences
• CPC Class Codes: C12N 2770/10022 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2770/10034 Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
• CPC Class Codes: C12N 7/00 Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00
• CPC Class Codes: C12Q 1/6883 for diseases caused by alterations of genetic material
• CPC Class Codes: C12Q 1/6888 for detection or identification of organisms
• CPC Class Codes: Y10S 424/815 Viral vaccine for porcine species, e.g. swine
• CPC Class Codes: Y10S 435/975 Kit

Abstract

Composition of matter comprising the causative agent of Mystery Swine Disease, Lelystad Agent, in a live, attenuated, dead, or recombinant form, or a part or component of it. Vaccine compositions and diagnostic kits based thereon. Recombinant nucleic acid comprising a Lelystad Agent-specific nucleotide sequence. Peptides comprising a Lelystad Agent-specific amino acid sequence. Lelystad Agent-specific antibodies.

Claims

1. A diagnostic kit for detecting an antibody in a biological sample from an animal which antibody specifically recognizes a causative agent of Mystery Swine Disease, said causative agent being antigenically cross-reactive with Lelystad Agent comprising a virus, said virus characterized in being sensitive to chloroform and having a size smaller than 200 nanometers, said virus further being immunoreactive with serum antibodies of a sow, said serum antibodies obtained by:

a) inoculating the sow with the virus deposited Jun. 5, 1991 with the Institut Pasteur, Paris, France, deposit number I-1102; and

b) collecting serum antibodies from the thus inoculated sow after 25 to 33 days, said diagnostic kit comprising

said causative agent; and

means for detecting said antibody from said biological sample.

2. The diagnostic kit of claim 1, wherein the causative agent is live.

3. The diagnostic kit of claim 1, wherein said means for detecting comprise an antibody detection assay.

4. The diagnostic kit of claim 1, wherein said means for detecting comprise an immunofluorescence assay.

5. The diagnostic kit of claim 1, wherein said means for detecting comprise an enzyme linked assay.

6. A process for detecting the presence of a causative agent of Mystery Swine Disease in an animal, said process comprising:

preparing a biological sample taken from the animal;

contacting said biological sample with antibodies immunoreactive with a causative agent of Mystery Swine Disease, said causative agent being antigenically cross-reactive with Lelystad Agent, said Lelystad Agent comprising a virus characterized in being sensitive to chloroform and having a size smaller than 200 nanometers, said virus further being immunoreactive with serum antibodies of a sow, said serum antibodies obtained by inoculating the sow with the virus deposited Jun. 5, 1991 with the Institut Pasteur, Paris, France, deposit number I-1102 and collecting serum antibodies from the thus inoculated sow after 25 to 33 days; and

detecting whether the biological sample contains said causative agent.

7. The process according to claim 6, wherein said biological sample is serum taken from the animal.