Polyunsaturated fatty acids in plants
First Claim
1. A method for producing stearidonic acid in a plant seed, said method comprising:
- growing a plant having integrated into its genome a first DNA construct comprising, in the 5′
to 3′
direction of transcription, a promoter functional in a plant seed cell, a DNA sequence encoding a delta-six desaturase, and a transcription termination region functional in a plant cell, and growing said plant under conditions whereby said delta-six desaturase is expressed.
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Abstract
The present invention relates to compositions and methods for preparing polyunsaturated long chain fatty acids in plants, plant parts and plant cells, such as leaves, roots, fruits and seeds. Nucleic acid sequences and constructs encoding fatty acid desaturases, including Δ5-desaturases, Δ6-desaturases and Δ12-desaturases, are used to generate transgenic plants, plant parts and cells which contain and express one or more transgenes encoding one or more desaturases. Expression of the desaturases with different substrate specificities in the plant system permit the large scale production of polyunsaturated long chain fatty acids such as docosahexaenoic acid, eicosapentaenoic acid, α-linolenic acid, gamma-linolenic acid, arachidonic acid and the like for modification of the fatty acid profile of plants, plant parts and tissues. Manipulation of the fatty acid profiles allows for the production of commercial quantities of novel plant oils and products.
144 Citations
12 Claims
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1. A method for producing stearidonic acid in a plant seed, said method comprising:
-
growing a plant having integrated into its genome a first DNA construct comprising, in the 5′
to 3′
direction of transcription, a promoter functional in a plant seed cell, a DNA sequence encoding a delta-six desaturase, and a transcription termination region functional in a plant cell, andgrowing said plant under conditions whereby said delta-six desaturase is expressed. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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Specification