Germination activated Ganoderma lucidum spores and method for producing the same

• US 6,468,542 B2
• Filed: 03/12/2001
• Issued: 10/22/2002
• Est. Priority Date: 10/12/1999
• Status: Expired due to Fees

• Current Assignee: Chee-Keung Chung, Xin Liu
• Original Assignee: Chee-Keung Chung, Xin Liu
• Inventors: Liu, Xin, Chung, Chee-Keung
• Primary Examiner(s): TATE, CHRISTOPHER ROBIN
• Application Number:
  US09/802,862
• Publication Number:
  US 20010031265A1
• Time in Patent Office: 589 Days
• Field of Search: 424/195.15
• US Class Current: 424/195.15
• CPC Class Codes: A61K 36/074 Ganoderma
• CPC Class Codes: A61P 1/16 for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
• CPC Class Codes: A61P 3/10 for hyperglycaemia, e.g. antidiabetics
• CPC Class Codes: A61P 31/04 Antibacterial agents
• CPC Class Codes: A61P 31/12 Antivirals
• CPC Class Codes: A61P 31/18 for HIV
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: A61P 37/02 Immunomodulators
• CPC Class Codes: A61P 39/02 Antidotes
• CPC Class Codes: A61P 39/06 Free radical scavengers or antioxidants
• CPC Class Codes: A61P 9/00 Drugs for disorders of the cardiovascular system
• CPC Class Codes: C12N 1/14 Fungi culture of mushrooms A01G18/00; as new plants A01H15/00; Culture media therefor

Abstract

The present invention describes a method for germination activating spores of Ganoderma lucidum to produce bioactive substances which has medicinal effects on patients with immunological disorders, cancer, AIDS, hepatitis, diabetes, and cardiovascular diseases, and can prevent or inhibit free radical oxidation and hepatotoxic effects. The method can be subdivided into three stages. At the first stage, a germination activation method is introduced which includes soaking the spores in a solution to induce germination, and placing the germination treated spores in a culture box to induce the synthesis of bioactive substances and softening of the cell walls of the spores. At the second stage, sporoderm-broken ganoderma spores are collected by treating the epispores with cell wall breaking enzymes and/or mechanical force. At the last stage, the bioactive substances are extracted from the sporoderm-broken spores by drying at low temperature followed by extraction.

Claims

1. A pharmaceutical preparation comprising

activation sporoderm-broken ganoderma spores and optionally a pharmaceutically acceptable carrier;

wherein said activated sporoderm-broken ganoderma spores are prepared by;

soaking ganoderma spores in a solution which is selected from the group consisting of water, saline, and a nutritional solution to cause the spores to germinate;

placing said germination-treated ganoderma spores in a culture box at a relative humidity of 65-98% and temperature of 18-48°

C. to cause the germinated ganoderma spores to activate; and

treating the germination activated ganoderma spores to break the pores to produce activated sporoderm-broken ganoderma spores.

2. The pharmaceutical preparation according to claim 1, wherein said ganoderma spores are soaked in the solution for 30 minutes to 8 hours at no more than 50°

C.

3. The pharmaceutical preparation according to claim 1, wherein said nutritional solution is at least one selected from the group consisting of coconut juice, malt extract, ganoderma sporocarp extract, ganoderma capillitia extract, culture solution containing biotin, and culture solution containing monobasic potassium phosphate and magnesium sulfate.

4. The pharmaceutical preparation according to claim 2, wherein said ganoderma spores are soaked in the solution for 2 to 4 hours.

5. The pharmaceutical preparation according to claim 2, wherein said ganoderma spores are soaked in the solution at 20 to 43°

C.

6. The pharmaceutical preparation according to claim 1, wherein said solution is 0.1-5 times the weight of said spores.

7. The pharmaceutical preparation according to claim 1, wherein the spores are broken by treating the germination activated ganoderma spores with an enzyme with a cell wall dissolving property.

8. The pharmaceutical preparation according to claim 7, wherein enzyme is chitinase or cellulase.

9. The pharmaceutical preparation according to claim 1, wherein the spores are broken by treating the germination activated ganoderma spores with a mechanical force.

10. The pharmaceutical preparation according to claim 9, wherein said mechanical force is at least one selected from the group consisting a micronization, roll pressing, grinding, ultrasound, and super high pressure microstream treatment.