Recombinant cell clones having increased stability and methods of making and using the same

  • US 6,475,725 B1
  • Filed: 06/02/1999
  • Issued: 11/05/2002
  • Est. Priority Date: 06/20/1997
  • Status: Expired due to Term
First Claim
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1. A method for preparing a recombinant factor VIII protein or polypeptide having factor VIII activity from a CHO cell culture wherein said CHO cells have been transformed to express recombinant factor VIII, comprising the steps ofmultiplying a CHO recombinant cell clone stable in serum- and protein-free medium, wherein purified, ultrafiltered soybean peptide is added to the medium, so as to obtain a cell culture, culturing said cell culture containing stable cells in a bioreactor so as to obtain a cell culture, and harvesting said recombinant polypeptide from a supernatant of said cell culture.

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