Methods for transforming plants to express Cry2Ab δ-endotoxins targeted to the plastids

  • US 6,489,542 B1
  • Filed: 11/04/1998
  • Issued: 12/03/2002
  • Est. Priority Date: 11/04/1998
  • Status: Expired due to Term
First Claim
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1. A plant comprising a nucleotide sequence encoding a Cry2Ab Bacillus thuringiensis δ

  • -endotoxin protein comprising a plant functional promoter sequence operably linked to a first polynucleotide sequence encoding a plastid transit peptide, which linked in-frame to a second polynucleotide sequence encoding said Cry2Ab Bacillus thuringiensis δ

    -endotoxin protein, said second polynucleotide sequence being operably linked to a plant functional 3′

    end transcription termination and polyadenylation sequence, wherein expression of said nucleotide sequence in said plant yields a fusion protein comprised of an amino-terminal plastid transit peptide covalently linked to said δ

    -endotoxin protein, and said plastid transit peptide functions to localize said δ

    -endotoxin protein to a plastid.

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