Methods and compositions for chromosome 21-specific staining
First Claim
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1. A method of staining interphase chromosome 21 DNA, comprising:
- (a) providing a chromosome 21-specific staining composition which comprises a heterogenous mixture of labeled unique sequence DNA fragments wherein said fragments are substantially complementary to DNA segments within human chromosome 21 DNA; and
(b) employing the heterogenous mixture and interphase chromosomal DNA in in situ hybridization to permit detection of labeled unique sequence DNA fragments which are hybridized to human chromosome 21 DNA, wherein the interphase chromosomal DNA is present in a morphologically identifiable cell nucleus during the in situ hybridization.
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Abstract
Methods and compositions for chromosome-specific staining are provided. Composition comprise heterogenous mixtures of labeled nucleic acid fragments having substantially complementary base sequences to unique sequence regions of the chromosomal DNA for which their associated staining reagent is specific. Methods include methods for making the chromosome-specific staining compositions of the invention, and methods for applying the staining compositions to chromosomes.
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16 Claims
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1. A method of staining interphase chromosome 21 DNA, comprising:
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(a) providing a chromosome 21-specific staining composition which comprises a heterogenous mixture of labeled unique sequence DNA fragments wherein said fragments are substantially complementary to DNA segments within human chromosome 21 DNA; and
(b) employing the heterogenous mixture and interphase chromosomal DNA in in situ hybridization to permit detection of labeled unique sequence DNA fragments which are hybridized to human chromosome 21 DNA, wherein the interphase chromosomal DNA is present in a morphologically identifiable cell nucleus during the in situ hybridization. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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