Polymerase chain reaction assays for monitoring antiviral therapy and making therapeutic decisions in the treatment of acquired immunodeficiency syndrome

  • US 6,503,705 B2
  • Filed: 02/13/2001
  • Issued: 01/07/2003
  • Est. Priority Date: 05/14/1992
  • Status: Expired due to Fees
First Claim
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1. A method of evaluating the effectiveness of anti-HIV therapy of an HIV-infected patient comprising:

  • a) collecting statistically significant data useful for determining whether or not a decline in plasma HIV RNA copy numbers exists after initiating treatment of an HIV-infected patient with an antiretroviral agent by;

    (i) collecting more than one plasma sample from the HIV-infected patient at time intervals sufficient to ascertain the existence of a statistically significant decline in plasma HIV RNA copy numbers;

    (ii) amplifying the HIV-encoding nucleic acid in the plasma samples using HIV primers via PCR for about 30 cycles;

    (iii) measuring HIV RNA copy numbers using the products of the PCR of step (ii);

    (iv) comparing the HIV RNA copy numbers in the plasma samples collected during the treatment; and

    b) evaluating whether a statistically significant decline in plasma HIV RNA copy numbers exists in evaluating the effectiveness of anti-HIV therapy of a patient.

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