Metering head for parallel processing of a plurality of fluid samples

• US 6,506,611 B2
• Filed: 02/05/2001
• Issued: 01/14/2003
• Est. Priority Date: 08/07/1998
• Status: Expired due to Fees

• Current Assignee: Deutsches Ressourcenzentrum Fur Genomforschung GmbH
• Original Assignee: Deutsches Ressourcenzentrum Fur Genomforschung GmbH
• Inventors: Bienert, Klaus, Kraack, Heiko, Zettl, Rolf, Vente, Andreas
• Primary Examiner(s): LUDLOW, JAN M
• Application Number:
  US09/776,742
• Publication Number:
  US 20010019845A1
• Time in Patent Office: 708 Days
• Field of Search: 422/100, 436/180, 738/641.7, 738/642.4, 738/642.5
• US Class Current: 436/180
• CPC Class Codes: B01L 3/021 Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
• CPC Class Codes: G01N 2035/00237 Handling microquantities of analyte, e.g. microvalves, capillary networks
• CPC Class Codes: G01N 2035/1069 by adjusting the spacing between multiple probes of a single transferring head
• CPC Class Codes: G01N 35/1067 for transfer to or from containers having different spacing
• CPC Class Codes: G01N 35/1072 with provision for selective pipetting of individual channels
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer
• CPC Class Codes: Y10T 436/25875 Gaseous sample or with change of physical state

Abstract

A freely traversable metering head with numerous metering devices, wherein the metering devices are each provided individually or block-by-block with an activating device, and wherein a controller traversable with the metering head is designed for the independent operation of one or more activating devices. Metering devices for such a metering head and procedures for their use are also disclosed.

Claims

1. A metering head that is freely movable using a device for traversing the metering head, the metering head comprising:

a plurality of metering devices, which, as individual metering devices or as metering device blocks, are provided with respective activating devices individually or in blocks, wherein the metering devices comprise picking needles or micropipettes arranged in accordance with orientation of sample volumes in a microtiter plate, the activating devices each being formed by a pneumatic cylinder having an actuating needle for activating the metering devices or metering device blocks, and

a controller traversable with the metering devices provided with control outputs whose number at least equals the number of pneumatic cylinders and which are connected with an accompanying control output by a pressure line, such that the metering devices or metering device blocks can be independently activated during metering head operation.

2. The metering head according to claim 1, in which the metering devices are arranged in a matrix and the pneumatic cylinders form an actuating matrix cooperating with the metering devices arranged in said matrix.

3. The metering head according to claim 1, in which the metering devices or metering device blocks form a metering station which is a detachable part the metering head as a replaceable module.

4. The metering head according to claim 3, in which the metering station includes an actuating matrix and a mounting block, to which picking needles or micropipettes are secured such that their upper end supports are oriented essentially in one plane, and ends of the actuating needles contact the actuating matrix.

5. The metering head according to claim 4, in which the metering devices are micropipettes secured to a carrier plate of the mounting block, each micropipette having a pipette piston, which is biased by a piston spring against the cylinder of the micropipette.

6. The metering head according to claim 5, in which the micropipettes can be axially moved relative to the carrier plate, and wherein the cylinder of the micropipette is biased by a cylinder spring against the carrier plate.

7. The metering head according to claim 6, in which a spring rate of the cylinder spring is less than a spring rate of the piston spring.

8. The metering head according to claim 7, in which, upon activation of an accompanying actuating needle, each micropipette can first be moved from a basic position into a pipetting position by compressing a corresponding cylinder spring, and then be activated to release pipetting output by compressing the corresponding piston spring.

9. The metering head according to claim 1, wherein 96 or 384 metering devices are provided.

10. The metering head according to claim 1 in which a plate element is provided between an actuating matrix and the metering devices, which connects end supports of block-wise arranged or all metering devices with each other.

11. The metering head according to claim 1 in which plate elements or block frames are provided, with which the metering devices are connected in blocks.

12. The metering head according to claim 11, in which an actuating matrix comprises the actuating needles whose number corresponds to a number of plate elements or block frames.

13. The metering head according to claim 1, further comprising a lid station and/or a gripper station.

14. The metering head according to claim 1, wherein the metering devices are micropipettes having a cylinder, a pipette tip, a pipette piston, a piston spring that biases the pipette piston against the cylinder, and a cylinder spring that biases the cylinder against a carrier, which forms a guide for the axial movement of the micropipette against tension of the cylinder spring.

15. The metering head according to claim 14, in which the cylinder spring has a lower spring rate than the piston spring.

16. A picking/spotting system equipped with a metering head according to claim 1.

17. A method of taking or releasing a plurality of fluid samples using a metering head according to claim 1, comprising individually or simultaneously activating metering devices to take samples from a carrier substrate or place them on the carrier substrate.

18. The method according to claim 17, comprising manipulating samples in substance libraries for molecular genetic, biochemical or medical purposes.

19. The method according to claim 17, comprising substantially simultaneously taking samples from a carrier substrate with all metering devices and sequentially placing said samples on a target substrate block-wise, and wherein the metering head traverses over a predetermined path after each sample deposition with one respective block of the metering devices.