Molecular cloning and expression of G-protein coupled receptors
DCFirst Claim
1. An isolated polynucleotide molecule selected from the group consisting of a polynucleotide which encodes a polypeptide comprising the amino acid sequence shown in SEQ ID NO. 16, and a polynucleotide which is complementary to a polynucleotide which encodes a polypeptide comprising the amino acid sequence shown in SEQ ID NO. 16.
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Abstract
The subject invention pertains to cloning and expression of novel cDNAs which encode members of the G-protein coupled receptor superfamily of proteins. Polynucleotides which encode mammalian H218 protein are described. The invention also concerns methods for screening for ligands of H218 protein. The proteins and peptides of the subject invention can also be used to produce antibodies which can bind to the subject proteins. The polynucleotide molecules, proteins, and antibodies of the subject invention can be used in both diagnostic and therapeutic applications.
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2 Claims
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1. An isolated polynucleotide molecule selected from the group consisting of a polynucleotide which encodes a polypeptide comprising the amino acid sequence shown in SEQ ID NO. 16, and a polynucleotide which is complementary to a polynucleotide which encodes a polypeptide comprising the amino acid sequence shown in SEQ ID NO. 16.
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2. An isolated polynucleotide comprising SEQ ID NO. 15.
Specification