Methods for improving sheath fluids and collection systems for sex-specific cytometer sorting of sperm

  • US 6,524,860 B1
  • Filed: 02/23/2000
  • Issued: 02/25/2003
  • Est. Priority Date: 12/31/1997
  • Status: Expired due to Term
First Claim
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1. A method of sorting cells by flow cytometry comprising the steps of:

  • a. establishing a cell source which supplies cells to be sorted;

    b. chemically coordinating a sheath fluid to create a sheath fluid environment for said cells which is coordinated with both a pre-sort and a post-sort cell fluid environment and combining said cells with said sheath fluid;

    c. sensing a property of said cells in a flow cytometer;

    d. discriminating between cells having a desired characteristic; and

    e. collecting cells having said desired characteristic.

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