Methods for detection of a target nucleic acid sequence

  • US 6,548,250 B1
  • Filed: 08/30/2000
  • Issued: 04/15/2003
  • Est. Priority Date: 10/29/1999
  • Status: Expired due to Term
First Claim
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1. A method of generating a signal indicative of the presence of a target nucleic acid sequence in a sample, comprising forming a cleavage structure comprising duplex and single-stranded nucleic acid, wherein said single stranded nucleic acid comprises a flap, by incubating a sample comprising a target nucleic acid sequence with a nucleic acid polymerase and cleaving said cleavage structure with a FEN nuclease to generate a signal, wherein generation of said signal is indicative of the presence of a target nucleic acid sequence in said sample.

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