Chemiluminescent substrates for neuraminidase, assays for detection of neuraminidase and kits therefor

  • US 6,555,698 B1
  • Filed: 11/16/1999
  • Issued: 04/29/2003
  • Est. Priority Date: 11/17/1998
  • Status: Expired due to Term
First Claim
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1. An enzymatically cleavable chemiluminescent 1,2-dioxetane of formula I:

  • embedded imagewhich is capable of producing light energy when decomposed;

    wherein T is a substituted or unsubstituted polycycloalkyl group bonded to the 4-membered ring portion of said dioxetane by a Spiro linkage;

    wherein X is an aryl or heteroaryl moiety of 6-30 carbon atoms which induces chemiluminescent decomposition of said 1,2-dioxetane upon enzymatic cleavage of said moiety Z;

    wherein Z is an enzymatically cleavable group of formula II;

    embedded image

    wherein R1-R3 are each independently H or an alkyl (branched or straight chain) of 1-4 carbon atoms, and wherein R is an alkyl, aryl, aralkyl or cycloalkyl of 1-20 carbon atoms.

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