High-density functional slide and preparation method thereof

• US 6,605,363 B2
• Filed: 04/18/2001
• Issued: 08/12/2003
• Est. Priority Date: 09/04/2000
• Status: Active Grant

• Current Assignee: Industrial Technology Research Institute
• Original Assignee: Industrial Technology Research Institute
• Inventors: Kuo, Wen-Hsun, Wu, Cheng-Tao, Tsao, Jia-Huey, Pan, Chao-Chi, Chang, Yao-Sung, Liu, Yu-Ching, Chow, Zu-Sho, Jan, Bor-Iuan, Ho, Chih-Wei
• Primary Examiner(s): Moore, Margaret G.
• Assistant Examiner(s): FEELY, MICHAEL J
• Application Number:
  US09/836,322
• Publication Number:
  US 20020028506A1
• Time in Patent Office: 846 Days
• Field of Search: 428/447, 428/448, 428/524, 428/428, 428/429
• US Class Current: 428/448
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00387 Applications using probes
• CPC Class Codes: B01J 2219/00529 DNA chips
• CPC Class Codes: B01J 2219/00576 fluorophore
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00641 the porous medium being continuous, e.g. porous oxide substrates
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C07B 2200/11 Compounds covalently bound to a solid support
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: Y10T 428/31612 As silicone, silane or siloxane
• CPC Class Codes: Y10T 428/31663 As siloxane, silicone or silane
• CPC Class Codes: Y10T 428/31667 Next to addition polymer from unsaturated monomers, or aldehyde or ketone condensation product
• CPC Class Codes: Y10T 428/31786 Of polyester [e.g., alkyd, etc.]
• CPC Class Codes: Y10T 428/31938 : Polymer of monoethylenically unsaturated hydrocarbon
• CPC Class Codes: Y10T 428/31942 : Of aldehyde or ketone condensation product

Abstract

The invention features a method for preparing a high-density functional slide by coating a sol-gel containing amine-group bearing silanes and a solution containing polyaldehyde groups onto an organic or inorganic substrate, respectively. The resulting slide is useful in the preparation of highly homogeneous functional-group slides and the high-density and high-efficiency bio-chip/microarray.

Claims

1. A high-density functional slide, comprising:

(i) a substrate;

(ii) an interlayer of a silane formed by coating a sol-gel of silanes onto said substrate; and

(iii) a polyaldehyde layer formed onto said interlayer.

2. The slide as claimed in claim 1, wherein the substrate comprises an organic or inorganic substrate.

3. The slide as claimed in claim 2, wherein the organic substrate comprises a polymer polymerized by organic monomers, wherein said organic monomers are selected from the group consisting of a monomer of ethylene, propylene, ester, acrylic acid, acrylate, alkyl acrylic acid, and alkyl acrylate.

4. The slide as claimed in claim 2, wherein the inorganic substrate is selected from the group consisting of silicon wafer, ceramic material, glass, and metal.

5. The slide as claimed in claim 1, wherein the polyaldehyde comprises a polyvinylalcohol-based polyaldehyde graft copolymer.

6. The slide as claimed in claim 5, wherein the polyvinylalcohol-based polyaldehyde graft copolymer is prepared by co-polymerization of allyl alcohol and acrolein using polyvinylalcohol as a stem nucleus.

7. The slide as claimed in claim 6, wherein the polyvinylalcohol-based polyaldehyde graft copolymer comprises an aldehyde density that is controlled by mixing different ratios of allyl alcohol to acrolein.

8. The slide as claimed in claim 1, wherein said sol-gel of silanes is polymerized by a monomer of silane in the presence of a catalyst.

9. The slide as claimed in claim 8, wherein the monomer comprises silane with amine group.

10. The slide as claimed in claim 8, wherein the monomer comprises mixtures of silane with and without an amine group.

11. The slide as claimed in claim 10, wherein the monomer of silane with an amine group comprises aminopropyl-triethoxysilane (APTES), and the monomer of silane without amine group comprises tetraethoxysilane (TEOS).

12. The slide as claimed in claim 10, wherein the mixtures of silane comprise an amine density that is controlled by mixing varying ratios of silane with an amine group to silane without an amine group.

13. The slide as claimed in claim 12, wherein the amine groups on said interlayer are covalently bonded to part of the aldehyde groups on said polyaldehyde layer.

14. The slide as claimed in claim 13, wherein part of the aldehyde groups on said polyaldehyde layer are unbonded to the amine groups on said interlayer, said unbonded aldehyde groups being exposed to form a functional layer on said slide with a distribution of high-density aldehyde groups.

15. The slide as claimed in claim 14, wherein the unbonded aldehyde groups on the slide are bonded to an amine group of a bio-molecule to be immobilized by forming a Schiff linkage.