Cell culture process

  • US 6,610,516 B1
  • Filed: 11/27/2000
  • Issued: 08/26/2003
  • Est. Priority Date: 04/26/1999
  • Status: Expired due to Term
First Claim
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1. A process for producing a human glycoprotein having multiple glycoforms, comprising culturing Chinese hamster ovary cells expressing nucleic acid encoding said glycoprotein in a serum-free medium in a production phase at a temperature of about 30°

  • C. to 35°

    C. and in the presence of about 0 to 2 mM of a butyrate salt, wherein the process produces an increased percentage of glycoprotein molecules having one glycoform relative to an identical process performed at 37°

    C. in the absence of butyrate.

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