Microfluidic systems and methods for determining modulator kinetics

  • US 6,613,580 B1
  • Filed: 06/30/2000
  • Issued: 09/02/2003
  • Est. Priority Date: 07/06/1999
  • Status: Active Grant
First Claim
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1. A method of determining the effect of a modulator on the interaction between at least two compounds at a plurality of modulator concentrations using a microfluidic device, the method comprising:

  • relying on the principles of Taylor dispersion and diffusion to estimate a concentration profile of the modulator in the flow direction of the modulator at a first detection point along a length of a first microchannel of the microfluidic device, the concentration profile including a plurality of modulator concentrations;

    introducing the interacting compounds into the first microchannel at a location upstream from said first detection point, at least a portion of the interacting compounds interacting in the presence of the modulator as they travel through the first microchannel;

    detecting the effect of the modulator on the interacting compounds at the first detection point thereby providing a modulation signal profile of the effect of the modulator on the interacting compounds; and

    correlating the estimated concentration profile of the modulator to the modulation signal profile to determine the effect of the modulator on the interaction between the two compounds at the plurality of modulator concentrations.

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