Systems for monitoring and controlling fluid flow rates in microfluidic systems

• US 6,616,823 B2
• Filed: 02/15/2001
• Issued: 09/09/2003
• Est. Priority Date: 07/15/1997
• Status: Expired due to Term

• Current Assignee: Caliper Holdings Incorporated
• Original Assignee: Caliper Holdings Incorporated
• Inventors: Kopf-Sill, Anne R.
• Primary Examiner(s): Nguyen, Nam
• Assistant Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US09/784,647
• Publication Number:
  US 20010020589A1
• Time in Patent Office: 936 Days
• Field of Search: 204/452, 204/454, 204/602, 204/600, 204/601, 422/70, 422/110, 422/50, 422/100, 422/105, 422/81, 422/68.1, 73/11.6, 738/610.7, 700/266
• US Class Current: 204/602
• CPC Class Codes: B01L 2200/143 Quality control, feedback systems
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2400/0418 electro-osmotic flow [EOF]
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/082 Active control of flow resistance, e.g. flow controllers
• CPC Class Codes: B01L 3/502746 characterised by the means for controlling flow resistance, e.g. flow controllers, baffles B01L3/502738 takes precedence
• CPC Class Codes: G01N 27/44752 Controlling the zeta potential, e.g. by wall coatings

Abstract

The present invention generally provides methods and systems for monitoring and controlling electroosmotic flow rates in microfluidic systems. Generally, such methods and systems monitor flow rates in electroosmotically driven microfluidic systems by flowing signaling elements within these channels and measuring the flow rate of these signals. The methods of monitoring flow rates are also applied to methods and systems for continuously monitoring and controlling these flow rates in electroosmotically driven microfluidic systems.

Claims

1. A system comprising:

a microfluidic device that comprises a first channel network that includes at least a first microfluidic channel;

a flow controller operably coupled to the channel network to control fluid movement through the channel network;

a signal detector in sensory communication with a second position of the at least first microfluidic channel;

a computer operably coupled to the flow controller and the signal detector for instructing operation of the flow controller and for receiving data from the signal detector, the computer being programmed to;

receive a user input flow rate value;

instruct the flow controller to inject a detectable signal into a first position in the first microfluidic channel at a first time;

receive a detect signal from the detector that corresponds to detection of the detectable signal at the second position at a second time in the first microfluidic channel;

determine a flow rate of fluid in the first microfluidic channel from a distance between the first and second positions and the first and second times; and

instruct the flow controller to increase or decrease a flow rate in the first microfluidic channel to achieve the user input flow rate value.

2. The system of claim 1, wherein the microfluidic channel network includes at least a second microfluidic channel that intersects and is in fluid communication with the first microfluidic channel.

3. The system of claim 2, wherein the first and second microfluidic channels have first and second fluids disposed therein, respectively, the first and second fluids having different mobilities through the first and second microfluidic channels, respectively.

4. The system of claim 1, wherein the detectable signal comprises an optically detectable signal, and the signal detector comprises an optical signal detector.

5. The system of claim 1, wherein the detectable signal comprises a fluorescent compound and the signal detector comprises a fluorescence detector.

6. The system of claim 1, wherein the flow controller comprises an electrokinetic flow controller.

7. The system of claim 1, wherein after the step of instructing the flow controller to increase or decrease the flow rate in the first microfluidic channel, the computer is programmed to repeat the steps of instructing injection of a detectable signal, determining the flow rate and instructing the flow controller to increase or decrease the flow rate.

8. In a computer that is operably coupled to a microfluidic system that includes a microfluidic device having a microfluidic channel network disposed therein, which network includes at lea t a first microfluidic channel, a detector in sensory communication with the first microfluidic channel and a flow controller operably coupled to the channel network to direct fluid movement through the channel network, a software program for:

receiving a use input flow rate value;

initiating an inject signal instructing the flow controller to inject a detectable signal into the first microfluidic channel at a first position;

receiving a detect signal from the detector;

calculating a flow rate of fluid in the first microfluidic channel; and

instructing the flow controller to increase or decrease the flow rate in the first microfluidic channel in order to match the user input flow rate value.

9. The program of claim 8, further comprising programming for repeating the initiating, receiving, and calculating steps.