Methods and compositions for preserving glucose level in blood specimens

• US 6,632,844 B1
• Filed: 03/23/2001
• Issued: 10/14/2003
• Est. Priority Date: 03/29/2000
• Status: Active Grant

• Current Assignee: Washington University In St Louis
• Original Assignee: Washington University In St Louis
• Inventors: Landt, Michael
• Primary Examiner(s): Witz, Jean C.
• Application Number:
  US09/816,493
• Time in Patent Office: 935 Days
• Field of Search: 435/2, 514/693
• US Class Current: 514/693
• CPC Class Codes: A61L 2/0088 Liquid substances
• CPC Class Codes: G01N 33/66 involving blood sugars, e.g. galactose
• CPC Class Codes: G01N 33/96 involving blood or serum control standard

Abstract

The invention relates to methods and compositions for preserving blood samples. In particular, a method for stabilizing glucose level in a blood sample is provided, which method comprises adding an effective amount of glyceraldehyde to a blood sample, whereby glucose level in said blood sample remains substantially constant for a period of time. Kits and combinations for stabilizing glucose level in a blood sample are also provided.

Claims

1. A method for stabilizing glucose level in a blood sample, which method comprises adding an effective amount of glyceraldehyde to a blood sample, whereby glucose level in said blood sample remains substantially constant for a period of time.

2. The method of claim 1, wherein the glyceraldehyde is a racemic mixture of d,1-glyceraldehyde.

3. The method of claim 1, wherein the glyceraldehyde is d-glyceraldehyde or 1-glyceraldehyde.

4. The method of claim 1, wherein the glyceraldehyde is 1-glyceraldehyde.

5. The method of claim 2, wherein the final concentration of the racemic mixture of d,1-glyceraldehyde in the blood sample is from about 0.9 to about 20 mM.

6. The method of claim 5, wherein the final concentration of the racemic mixture of d,1-glyceraldehyde in the blood sample is from about 5 to about 10 mM.

7. The method of claim 4, wherein the final concentration of the 1-glyceraldehyde in the blood sample is from about 0.65 to about 10 mM.

8. The method of claim 7, wherein the final concentration of the 1-glyceraldehyde in the blood sample is from about 2.5 to about 5 mM.

9. The method of claim 1, wherein the blood sample is a whole blood, plasma or serum sample.

10. The method of claim 9, wherein the whole blood sample is a heparinized whole blood sample.

11. The method of claim 1, wherein the starting glucose concentration in the blood sample is from about 0 to about 100 mM.

12. The method of claim 11, wherein the starting glucose concentration in the blood sample is from about 3.9 mM to about 13.4 mM.

13. The method of claim 1, wherein the starting glucose concentration does not decrease by more than 5%.

14. The method of claim 1, wherein the starting glucose concentration does not decrease by more than 2%.

15. The method of claim 1, wherein the glucose level in the blood sample remains substantially constant for at least about 16 hours.

16. The method of claim 1, wherein the glucose level in the blood sample remains substantially constant for at least about 8 hours.

17. The method of claim 1, wherein the blood sample with the added glyceraldehyde is subjected to a metabolism assay.

18. The method of claim 17, wherein the metabolism assay is for an analyte selected from the group consisting of an ion, glucose, creatinine, urea, bilirubin, albumin, alkaline phosphatase activity, aspartate aminotransferase, total protein and total CO₂.

19. The method of claim 18, wherein the ion to be assayed is selected from the group consisting of a sodium, a potassium, a chloride and a calcium ion.

20. The method of claim 17, wherein the metabolism assay is operated on an automated analyzer.

21. The method of claim 20, wherein the automated analyzer is selected from the group consisting of Cobas-MIRA analyzer, Ortho-Clinical Diagnostics Vitros 250, Dade Behring RxL and Hitachi 747 analyzer.

22. A method for stabilizing glucose level in a blood sample, which method comprises adding an effective amount of glyceraldehyde and an effective amount of an anti-glycolytic agent that is not glyceraldehyde to a blood sample, whereby glucose level in said blood sample remains substantially constant for a period of time.

23. A method for preserving a blood sample for a further analysis, which method comprises adding an effective amount of glyceraldehyde and an effective amount of an a blood-preserving agent to a blood sample, whereby the quality of said blood sample is substantially preserved for a further analysis.