Methods and reagents for the rapid and efficient isolation of circulating cancer cells

• US 6,645,731 B2
• Filed: 07/13/2001
• Issued: 11/11/2003
• Est. Priority Date: 02/12/1998
• Status: Expired due to Term

• Current Assignee: Menarini Silicon Biosystems S.P.A
• Original Assignee: Immunivest Corporation
• Inventors: Uhr, Jonathan W., Racila, Emilian V., Rao, Galla Chandra, Terstappen, Leon W. M. M., Liberti, Paul A.
• Primary Examiner(s): HUFF, SHEELA JITENDRA
• Application Number:
  US09/904,472
• Publication Number:
  US 20020009759A1
• Time in Patent Office: 851 Days
• Field of Search: 435/7.1, 435/7.21, 435/7.23, 436/64, 436/813
• US Class Current: 435/7.23
• CPC Class Codes: B03C 1/01 by addition of magnetic adjuvants
• CPC Class Codes: B82Y 25/00 Nanomagnetism, e.g. magnetoimpedance, anisotropic magnetoresistance, giant magnetoresistance or tunneling magnetoresistance
• CPC Class Codes: G01N 33/574 for cancer
• CPC Class Codes: G01N 33/57484 involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
• CPC Class Codes: G01N 33/57492 involving compounds localized on the membrane of tumor or cancer cells
• CPC Class Codes: H01F 1/0054 Coated nanoparticles, e.g. nanoparticles coated with organic surfactant

Abstract

A highly sensitive assay is disclosed which combines immunomagnetic enrichment with multiparameter flow cytometric and immunocytochemical analysis to detect, enumerate and characterize carcinoma cells in the blood. The assay can detect one epithelial cell or less in 1 ml of blood and has a greater sensitivity than conventional PCR or immunohistochemistry by 1-2 orders of magnitude. In addition, the assay facilitates the biological characterization and staging of carcinoma cells.

Claims

1. A method for determining likelihood of cancer recurrence in a human subject previously treated for cancer, comprising:

a) obtaining a blood sample from said subject;

b) determining the number of epithelial cells in said sample;

c) comparing the number of epithelial cells with a statistically determined number of epithelial cells from a group of tumor-free patient controls; and

d) assigning a likelihood of cancer recurrence when the number of epithelial cells exceeds a pre-determined value based on statistical averages of circulating epithelial cells from healthy subjects compared with statistical averages of circulating epithelial cells from cancer patients.

2. The method of claim 1 further comprising identifying the tissue origin of the epithelial cells.

3. The method of claim 2 wherein tissue origin of the epithelial cells is identified by cell surface binding with selected tumor specific antibodies.

4. The method of claim 1 wherein tumor recurrence is determined to be likely when epithelial cell number in blood is about 5 to about 15 epithelial cells per 20 ml of blood.

5. A method of distinguishing an organ confined carcinoma from a carcinoma with metastatic properties, comprising:

a) obtaining a blood sample from a test subject;

b) determining the number of circulating epithelial cells in said sample;

c) identifying the tissue origin of the epithelial cells;

d) comparing the number of circulating tissue specific epithelial cells determined for said test subject with a statistically determined average number of circulating tissue specific epithelial cells from a group of healthy subjects, a group of organ-confined carcinoma patients and a group of patients with metastatic carcinoma; and

e) identifying said human test subject cells as metastatic when the number is within the statistically determined average for the group of patients with metastatic carcinoma.

6. The method of claim 5 wherein the carcinoma is a carcinoma selected from the group of consisting of prostate cancer, breast cancer, colon cancer, apudoma, choristoma, branchioma, malignant carcinoid syndrome, carcinoid heart disease, Walker, basal cell, basosquamous, Brown-Pearce, ductal, Ehrlich tumor, in situ, Krebs 2, merkel cell, mucinous, non-small cell lung, oat cell, papillary, scirrhous, bronchiolar, bronchogenic, squamous cell and transitional cell reticuloendotheliosis, melanoma, chondroblastoma, chondroma, chondrosarcoma, fibroma, fibrosarcoma, giant cell tumors, histiocytoma, lipoma, liposarcoma, mesothelioma, myxoma, myxosarcoma, osteoma, osteosarcoma, Ewing'"'"'s sarcoma, synovioma, adenofibroma, adenolymphoma, carcinosarcoma, chordoma, mesenchymoma, mesonephroma, myosarcoma, ameloblastoma, cementoma, odontoma, teratoma, throphoblastic tumor, adenocarcinoma, adenoma, cholangioma, cholesteatoma, cylindroma, cystadenocarcinoma, cystadenoma, granulosa cell tumor, gynandroblastoma, hepatoma, hidradenoma, islet cell tumor, leydig cell tumor, papilloma, sertoli cell tumor, theca cell tumor, leiomyoma, leiomyosarcoma, myoblastoma, myoma, myosarcoma, rhabdomyoma, rhabdomyosarcoma, ependymoma, ganglioneuroma, glioma, medulloblastoma, meningioma, neurilemmoma, neuroblastoma, neuroepithelioma, neurofibroma, neuroma, paraganglioma, paraganglioma nonchromaffin, antiokeratoma, angioma sclerosing, angiomatosis, glomangioma, hemangioendothelioma, hemangioma, hemangiopericytoma, hemangiosarcoma, lymphangioma, lymphangiomyoma, lymphangiosarcoma, pinealoma, carcinosarcoma, chondrosarcoma, cystosarcoma phyllodes, fibrosarcoma, hemangiosarcoma, leiomyosarcoma, leukosarcoma, liposarcoma, lymphangiosarcoma, myosarcoma, myxosarcoma, ovarian carcinoma, rhabdomyosarcoma, Kaposi'"'"'s sarcoma, mast-cell sarcoma, and neoplasms selected from the group consisting of bone, digestive system, liver, pancreatic, pituitary, testicular, orbital, head and neck, central nervous system, acoustic, pelvic, respiratory tract, and urogenital neoplasms, neurofibromatosis, and cervical dysplasia.

7. A method as claimed in claim 5 wherein the carcinoma is a breast carcinoma.

8. A method as claimed in claim 7 wherein a number of epithelial cells in the human test subject'"'"'s blood greater than the average number in a group of organ-confined breast carcinoma patients is indicative of metastatic breast carcinoma.

9. A method as claimed in claim 7 wherein a number of epithelial cells in the human test subject'"'"'s blood which is higher than average number in a group of healthy subjects but less than the average number in a group of metastatic breast carcinoma patients is indicative of organ-confined breast carcinoma.

10. A method of following remission status in a human cancer patient undergoing cancer therapy treatment, comprising:

a) obtaining a blood sample from a human cancer patient;

b) determining the number of circulating epithelial cells in said sample prior to and periodically subsequent to said cancer therapy treatment wherein an increase over time in the number of epithelial cells following treatment compared to the number of epithelial cells just after treatment is indicative of relapse and wherein maintenance of epithelial cell levels comparable to levels just after treatment is indicative of continuing remission.

11. The method of claim 10 wherein the carcinoma is a carcinoma selected from the group of consisting of prostate cancer, breast cancer, colon cancer, apudoma, choristoma, branchioma, malignant carcinoid syndrome, carcinoid heart disease, Walker, basal cell, basosquamous, Brown-Pearce, ductal, Ehrlich tumor, in situ, Krebs 2, merkel cell, mucinous, non-small cell lung, oat cell, papillary, scirrhous, bronchiolar, bronchogenic, squamous cell and transitional cell reticuloendotheliosis, melanoma, chondroblastoma, chondroma, chondrosarcoma, fibroma, fibrosarcoma, giant cell tumors, histiocytoma, lipoma, liposarcoma, mesothelioma, myxoma, myxosarcoma, osteoma, osteosarcoma, Ewing'"'"'s sarcoma, synovioma, adenofibroma, adenolymphoma, carcinosarcoma, chordoma, mesenchymoma, mesonephroma, myosarcoma, ameloblastoma, cementoma, odontoma, teratoma, throphoblastic tumor, adenocarcinoma, adenoma, cholangioma, cholesteatoma, cylindroma, cystadenocarcinoma, cystadenoma, granulosa cell tumor, gynandroblastoma, hepatoma, hidradenoma, islet cell tumor, leydig cell tumor, papilloma, sertoli cell tumor, theca cell tumor, leiomyoma, leiomyosarcoma, myoblastoma, myoma, myosarcoma, rhabdomyoma, rhabdomyosarcoma, ependymoma, ganglioneuroma, glioma, medulloblastoma, meningioma, neurilemmoma, neuroblastoma, neuroepithelioma, neurofibroma, neuroma, paraganglioma, paraganglioma nonchromaffin, antiokeratoma, angioma sclerosing, angiomatosis, glomangioma, hemangioendothelioma, hemangioma, hemangiopericytoma, hemangiosarcoma, lymphangioma, lymphangiomyoma, lymphangiosarcoma, pinealoma, carcinosarcoma, chondrosarcoma, cystosarcoma phyllodes, fibrosarcoma, hemangiosarcoma, leiomyosarcoma, leukosarcoma, liposarcoma, lymphangiosarcoma, myosarcoma, myxosarcoma, ovarian carcinoma, rhabdomyosarcoma, Kaposi'"'"'s sarcoma, mast-cell sarcoma, and neoplasms selected from the group consisting of bone, digestive system, liver, pancreatic, pituitary, testicular, orbital, head and neck, central nervous system, acoustic, pelvic, respiratory tract, and urogenital neoplasms, neurofibromatosis, and cervical dysplasia.