Chemical sensor and use thereof

• US 6,706,160 B2
• Filed: 03/12/2001
• Issued: 03/16/2004
• Est. Priority Date: 01/03/1995
• Status: Expired due to Fees

• Current Assignee: Chemel AB (Biotech-IgG Equity AB)
• Original Assignee: Chemel AB (Biotech-IgG Equity AB)
• Inventors: Kriz, Dario
• Primary Examiner(s): Tung, T.
• Application Number:
  US09/803,846
• Publication Number:
  US 20010023832A1
• Time in Patent Office: 1,100 Days
• Field of Search: 204/403, 204/415, 204/403.01, 204/403.06, 204/403.07, 204/403.09, 204/403.1, 204/403.14, 205/777.5, 205/778, 205/782, 205/782.5, 205/783, 205/787, 205/787.5
• US Class Current: 204/415
• CPC Class Codes: G01N 27/3271 Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001
• CPC Class Codes: G01N 33/143 containing sugar

Abstract

A chemical sensor selectively detects an analyte in a solution. The sensor comprises a flow-through chamber, a selective membrane, a transducer, an inlet for a liquid flow containing a recognition element, and an outlet. To detect an analyte in a solution, a recognition element is contacted with the solution containing the analyte via a selective membrane. This results in a response detectable by a transducer. The recognition element is injected into a flow, and the flow is passed into a flow-through chamber comprising the transducer and the selective membrane, where it contacts the analyte passing from the solution outside the chamber. The recognition element and the analyte interact to provide a signal that is detected by the transducer. The chemical sensor is used for detecting analyte(s) in a reactor system, a flow system or in an in vivo system.

Claims

1. A sensor, based on a dynamic initial change measuring process, for detecting an analyte in a solution, the sensor comprising:

a working electrode;

a selective membrane having first and second sides, the working electrode and the first side being spaced apart from each other to form

a flow-through chamber therebetween;

an inlet to the chamber;

an outlet from the chamber;

a source of buffer;

a source of recognition element;

an injector connected to each of said sources for selecting the buffer or the recognition element;

a pump connected to injector for pumping the selected one of the buffer and the recognition element through the inlet, the chamber and the outlet;

the second side being in contact with the solution so that analyte passes through the membrane and reacts with the recognition element, and the working electrode generating

a dynamic initial change signal proportional to the concentration of the analyte.

2. A sensor according to claim 1 wherein the working electrode is an electrochemical electrode.

3. A sensor according to claim 1 wherein the selective membrane is selected from the group consisting of ion exchange, gas permeable, group analyte selective hindering, and single analyte selective hindering membranes.

4. A sensor according to claim 1 wherein the recognition element is of biological origin.

5. A sensor according to claim 4 wherein the recognition element is catalytic.

6. A sensor according to claim 4 wherein the recognition element is non-catalytic.