Bio-chip, photoluminescent methods for identifying biological material, and apparatuses for use with such methods and bio-chips
First Claim
1. A tagging-free method to detect the binding of untagged single stranded nucleic acid to an untagged material of interest, comprising the steps of:
- (A) providing a sensor comprised of a first layer and a second layer wherein said first layer comprises an untagged single stranded nucleic acid and wherein said second layer comprises a photoluminescent material, and wherein said first layer and said second layer are separate layers, and wherein said second layer comprises material selected from the group consisting of aromatic polymers, doped or undoped metal oxides, sulfides, arsenides, tellurides, and nitride and phosphide nanocomposites;
(B) exposing said sensor to a biological sample for sufficient time for said untagged single stranded nucleic acid to bind to an untagged material of interest in said biological sample;
(C) applying light to said sensor; and
(D) measuring photoluminescence from said sensor, wherein photoluminescence measured in said step of exposing is indicative of binding of said untagged single stranded nucleic acid to said untagged material of interest.
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Abstract
A method detects binding of molecules, advantageously without tagging molecules in the sample. A sensor is used in which is included a single stranded nucleic acid sequence and a photoluminescent material in respective layers. After the sensor is exposed to a biological sample for sufficient time for its single stranded nucleic acid sequence to bind to a material of interest, photoluminescence from the sensor can be measured. An apparatus for tagging-free detection of binding of molecules also is provided. Methods of making tagging-free sensors are provided. Also, tagging-free methods to detect binding of antigens and related devices are disclosed.
31 Citations
22 Claims
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1. A tagging-free method to detect the binding of untagged single stranded nucleic acid to an untagged material of interest, comprising the steps of:
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(A) providing a sensor comprised of a first layer and a second layer wherein said first layer comprises an untagged single stranded nucleic acid and wherein said second layer comprises a photoluminescent material, and wherein said first layer and said second layer are separate layers, and wherein said second layer comprises material selected from the group consisting of aromatic polymers, doped or undoped metal oxides, sulfides, arsenides, tellurides, and nitride and phosphide nanocomposites;
(B) exposing said sensor to a biological sample for sufficient time for said untagged single stranded nucleic acid to bind to an untagged material of interest in said biological sample;
(C) applying light to said sensor; and
(D) measuring photoluminescence from said sensor, wherein photoluminescence measured in said step of exposing is indicative of binding of said untagged single stranded nucleic acid to said untagged material of interest. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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Specification