Process for preparing an electrochemical test strip

• US 6,723,371 B2
• Filed: 05/31/2001
• Issued: 04/20/2004
• Est. Priority Date: 06/01/2000
• Status: Active Grant

• Current Assignee: Bioptik Technology, Inc.
• Original Assignee: Bioptik Technology, Inc.
• Inventors: Chih-hui, Lee
• Primary Examiner(s): Tung, T.
• Assistant Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US09/871,350
• Publication Number:
  US 20020003087A1
• Time in Patent Office: 1,055 Days
• Field of Search: 204/403.01, 204/403.14, 204/403.06, 204/403.09, 427/2.13
• US Class Current: 427/2.13
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52

Abstract

As embodied and broadly described herein, the invention provides an electrode test strip. The electrochemical electrode test strip comprises an insulating base plate, a electrode system on the base plate, a spacer which partially covers the electrode system and a channel trench with a constant width is formed thereof, a reactive film, and a cover on the spacer with an first opening thereof. Wherein the electrode system comprises at least a working electrode and a reference electrode, and the working electrode and the reference electrode is isolated. The reactive film contains at least on active species that can have a specific redox reaction with the analyte. The first opening exposes the channel trench, and two second openings are located at the two ends of the channel trench.

Claims

1. A method of manufacturing a electrode test strip, comprising the steps of:

offering an insulating base plate;

printing a layer of conductive films to be a cathode and an anode, e cathode and the anode are isolated;

forming a first spacer and a second spacer on the conductive films to expose two ends of the cathode and the anode, wherein a channel trench is formed between the first and the second spacer;

spreading a reagent solution on a bottom surface of the channel trench;

drying the reagent solution to form a reactive film in the channel trench; and

forming a cover with a first opening on the spacer, wherein the first opening is located on the channel trench and two second openings are surrounded by the insulating base plate, the first spacer, the second spacer and the cover.

2. The method of the claim 1, wherein the reagent solution comprises an active species, a polymer, an electron medium, a nonionic surfactant and a buffer solution.

3. The method of the claim 1, wherein the polymer is about 1-4 wt % of the reagent solution.

4. The method of the claim 2, wherein the polymer is selected from the group consisting of Polyvinyl Pyrrolidone, Polyethylene Glycol, gelatin, dextran, polyvinyl alcohol, methylcellulose, carboxymethyl cellulose, albumin, chitin and a combination thereof.

5. The method of the claim 2, wherein the electron medium is about 1-10 wt % of the reagent solution.

6. The method of the claim 2, wherein the electro medium includes potassium ferricyanide.

7. The method of the claim 2, wherein the nonionic surfactant is less than about 0.3 wt % of the reagent solution.

8. The method of the claim 2, wherein the nonionic surfactant is selected from the group consisting of Triton X-100, lecithine, phosphatidyl choline, oleic acid, cyclodextrin and polyoxyethylene glycerine fatty acid ester.

9. The method of the claim 2, wherein the buffer solution is about 83.7-98 wt % of the reagent solution.

10. The method of the claim 2, wherein the buffer reagent of the buffer solution is a selected from the group consisting of citric acid, dipotassium phosphate, potassium phosphate, Tris and borate.

11. The method of the claim 2, wherein between the steps of drying the reagent solution and forming a cover further comprises a step of covering a nitrate fiber film on the channel trench.