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Biosensor with peroxidase enzyme

  • US 6,767,441 B1
  • Filed: 07/31/2001
  • Issued: 07/27/2004
  • Est. Priority Date: 07/31/2001
  • Status: Expired
First Claim
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1. A disposable electrode strip for measuring an analyte in a fluid sample said strip comprising:

  • a laminated strip having a first strip end, a second strip and and a vent opening spaced from said first strip and, said laminated strip comprising a base layer with at least two electrodes delineated thereon, a reagent holding layer carried on said base layer, said reagent holding layer having at least two cutouts, a channel forming layer carried on said reagent holding layer, and a cover, an enclosed channel between said first strip end and said vent opening, said enclosed channel containing said at least two cutouts;

    a first disposed in a first cutout of said at least two cutouts forming a reference electrode, said first reagent comprising a reference electrode material selected from the group consisting of silver chloride when said reference electrode is silver and a mixture made by combining at least a redox mediator and at least one binder when said reference electrode is selected from the group consisting of gold, gold/tin oxide, palladium, platinum and carbon composition;

    a second reagent disposed in a second cutout of said at least two cutouts forming a first working electrode, said second reagent comprising a redox mediator, at least one binder, at least one enzyme that is a substrate of said analyte and a peroxidase capable of catalyzing a reaction involving said redox mediator wherein said redox mediator is oxidized; and

    conductive contacts at said second strip end and insulated from said enclosed channel.

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