Nanoparticle-based sensors for detecting analytes in fluids

• US 6,773,926 B1
• Filed: 09/25/2001
• Issued: 08/10/2004
• Est. Priority Date: 09/25/2000
• Status: Active Grant

• Current Assignee: California Institute of Technology
• Original Assignee: California Institute of Technology
• Inventors: Lewis, Nathan S., Freund, Michael S., Briglin, Shawn M.
• Primary Examiner(s): Gakh, Yelena G.
• Application Number:
  US09/963,788
• Time in Patent Office: 1,050 Days
• Field of Search: 436/149, 436/151, 436/183, 436/119, 436/120, 436/111, 436/73, 436/80
• US Class Current: 436/149
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: G01N 27/126 comprising organic polymers
• CPC Class Codes: G01N 31/22 using chemical indicators G01N31/02 takes precedence
• CPC Class Codes: G01N 33/84 involving inorganic compounds or pH
• CPC Class Codes: Y10T 436/173845 Amine and quaternary ammonium
• CPC Class Codes: Y10T 436/18 Sulfur containing
• CPC Class Codes: Y10T 436/182 Organic or sulfhydryl containing [e.g., mercaptan, hydrogen, sulfide, etc.]

Abstract

Sensors, sensor arrays and sensing methods provide for detection of a chemical analyte in a fluid. Sensors include a plurality of conductive and nonconductive regions in contact with a measuring apparatus. One or more sensors include a plurality of particles that include a metallic core. Preferably, the particles also include one or more capping ligands coupled to the metallic core. Exposure of the sensors to a fluid containing a chemical analyte causes the analyte to react with the metal core, preferably by displacing one or more of the capping ligands. The chemical analyte can be detected based on a change in electrical or optical properties of the sensors.

Claims

1. A method of detecting a chemical analyte in a fluid, comprising:

providing one or more sensors comprising regions of conductive metal material and regions-of nonconductive material proximate to the conductive metal material, the nonconductive material including one or more chemical groups comprising one or more ligans coupled to the conductive metal material, the chemical groups being displaceable by a chemical analyte;

exposing the sensors to a fluid containing the chemical analyte under conditions sufficient to cause the chemical analyte to displace one or more of the chemical groups;

measuring a response based on the displacement of the chemical groups; and

detecting the chemical analyte based on the measured response.

2. The method of claim 1, wherein:

the conductive metal material includes a plurality of particles comprising one more metals.

3. The method of claim 2, wherein:

the particles include a metal core; and

the ligands are covalently coupled to the metal core.

4. The method of claim 3, wherein:

the chemical analyte is a thiol;

the metal core includes gold or silver; and

the chemical groups include an alkylamine.

5. The method of claim 3, wherein:

the particles are disposed in a polymer matrix.

6. The method of claim 5, wherein:

the polymer matrix includes a conducting, semiconducting, or insulating organic polymer.

7. The method of claim 3, wherein:

the chemical analyte has a size that is smaller than a size of one or more of the chemical groups; and

measuring the response includes measuring a decrease in resistance or an increase in conductivity resulting from displacement of the chemical groups by the chemical analyte.

9. The method of claim 3, wherein:

the ligands include one or more members selected from the group consisting of alkylamines, alkanethiols, phosphines, carboxylates, thiolates, nitrites, sulfonates and surfactants.

10. The method of claim 1, wherein:

measuring the response includes measuring a change in conductivity, resistance, impedance, capacitance, inductance, or optical properties of one or more of the sensors, or a combination thereof, upon exposure of the sensors to the chemical analyte.

11. The method of claim 1, wherein:

the nonconductive material includes a plurality of chemical groups coupled to the conductive metal material.

12. The method of claim 11, wherein:

the plurality of chemical groups includes a plurality of different chemical groups.

13. The method of claim 11, wherein:

the sensors are exposed to the fluid containing the chemical analyte under conditions sufficient to cause the chemical analyte to displace a first portion of the plurality of chemical groups, such that the conductive metal material remains coupled to one or more remaining portions of the plurality of chemical groups;

the method further comprising detecting a second chemical analyte by exposing the sensors to a fluid containing the second chemical analyte under conditions sufficient to cause the second chemical analyte to displace one or more remaining portions of the plurality of chemical groups, measuring a response based on the displacement of the one or more remaining portions of the plurality of chemical groups, and detecting the second chemical analyte based on the measured response.

14. The method of claim 13, wherein:

the second chemical analyte is the same as the chemical analyte.

15. The method of claim 13, wherein:

the second chemical analyte is different from the chemical analyte.

16. The method of claim 1, wherein:

the sensors include a plurality of different sensors.

17. The method of claim 16, wherein:

the plurality of different sensors form a sensor array.

18. The method of claim 17, wherein:

the sensor array includes two or more sensors each including a different conductive metal material.

19. The method of claim 17, wherein:

the sensor array includes two or more sensors each including a different chemical group.

20. The method of claim 17, wherein:

detecting the chemical analyte based on the measured response includes identifying the chemical analyte based on a different response measured for each of a plurality of the different sensors.

8. The method of claimed 3, wherein:

the metals include one or more of Ag, Au, Cu, Pt, Pd, Ni, W, Rh, Cr and alloys and mixtures thereof.

21. A method for detecting a chemical analyte in a fluid, comprising:

providing one or more sensors comprising a plurality of conductive metal particles disposed in a nonconductive matrix, the conductive metal particles comprising a metal core bounded to a chemical group;

exposing the sensors to a fluid-containing the chemical analyte under conditions sufficient to cause the chemical analyte to react with the metal particle 5 to form a capped particle metal cores by displacing the chemical groups with the chemical analyte;

measuring a response based on the reaction of the chemical analyte and the metal particle 5; and

detecting the chemical analyte based on the measured response.