Biological method for the production of adipic acid and intermediates
First Claim
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1. A method for the production of adipic acid comprising:
- contacting a transformed host cell under suitable growth conditions with an effective amount of cyclohexanol whereby adipic acid is produced, said transformed host cell comprising a nucleic acid fragment as set forth in SEQ ID NO;
27.
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Abstract
A gene cluster has been isolated from an Acinetobacter sp. that encodes the enzymes expected to convert cyclohexanol to adipic acid. The entire gene cluster has been cloned and all open reading frames have been sequenced. Cosmid clones have been identified containing the gene cluster. Demonstration of conversion of cyclohexanol to adipic acid has been made with the recombinant E. coli host strain containing the cosmids.
23 Citations
3 Claims
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1. A method for the production of adipic acid comprising:
- contacting a transformed host cell under suitable growth conditions with an effective amount of cyclohexanol whereby adipic acid is produced, said transformed host cell comprising a nucleic acid fragment as set forth in SEQ ID NO;
27. - View Dependent Claims (2, 3)
- contacting a transformed host cell under suitable growth conditions with an effective amount of cyclohexanol whereby adipic acid is produced, said transformed host cell comprising a nucleic acid fragment as set forth in SEQ ID NO;
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Current AssigneeE. I. du Pont de Nemours and Company (Corteva, Inc.)
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Original AssigneeE. I. du Pont de Nemours and Company (Corteva, Inc.)
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InventorsCheng, Qiong, Nagarajan, Vasantha, Thomas, Stuart M.
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Primary Examiner(s)HUTSON, RICHARD G
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Application NumberUS10/272,419Publication NumberTime in Patent Office706 DaysField of Search435/142, 435/183, 435/189, 435/190, 435/195, 435/1, 536/23.1, 536/23.2US Class Current435/142CPC Class CodesC12N 15/52 Genes encoding for enzymes ...C12P 7/44 Polycarboxylic acids
