Device for the take-up and/or release of liquid samples

• US 6,824,024 B2
• Filed: 10/24/2003
• Issued: 11/30/2004
• Est. Priority Date: 11/17/2000
• Status: Expired due to Term

• Current Assignee: Tecan Trading AG (Tecan Group AG)
• Original Assignee: Tecan Trading AG (Tecan Group AG)
• Inventors: Schmid, Noa, Ingenhoven, Nikolaus
• Primary Examiner(s): Mancene, Gene
• Assistant Examiner(s): Nicolas, Frederick C.
• Application Number:
  US10/416,785
• Publication Number:
  US 20040050866A1
• Time in Patent Office: 403 Days
• Field of Search: 222/1, 222/504, 222/509-510, 222/420, 222/524, 222/525, 222/482, 222/478, 222/386, 422/99-100, 738/640.1, 738/641.1, 738/643.3, 738/633.2
• US Class Current: 222/504
• CPC Class Codes: B01L 13/02 for receptacle or instruments
• CPC Class Codes: B01L 2400/025 tapping tip on substrate
• CPC Class Codes: B01L 2400/0439 ultrasonic vibrations, vibrating piezo elements
• CPC Class Codes: B01L 2400/0478 pistons
• CPC Class Codes: B01L 3/022 Capillary pipettes, i.e. having very small bore B01L3/0224 - B01L3/0237 take precedence
• CPC Class Codes: B01L 3/0241 Drop counters; Drop formers making arrays for combinatorial libraries B01J19/0046; automation of dispensing for analysis G01N35/10
• CPC Class Codes: G01N 2035/103 using disposable tips
• CPC Class Codes: G01N 35/10 Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices G01N35/0099 takes precedence

Abstract

A device for aspirating and/or dispensing liquid samples has a hollow body with a cavity for the liquid. A movement element for aspirating and/or dispensing the liquid is moved by a movement unit having a first drive element driven by a first drive. A pipette tip or adapter therefor, receives liquid from the cavity over a line, and a flush line discharges into the cavity. A carrier unit movably carries the movement unit in at least one spatial axis and includes at least one second drive element driven by a second drive. The movement unit includes the hollow body, the movement element, the first drive element, the second drive element and the pipette tip or adapter, as well as a pulse generator for generating pressure waves in the liquid in the cavity during dispensing, all these elements being movable together with respect to the carrier unit.

Claims

1. A device for at least one of aspirating and dispensing samples of a liquid with a movement unit, the device comprising:

a hollow body having a cavity adapted to be essentially filled with a system liquid or a sample liquid, and having a movement element, acting on the cavity, for at least one of aspirating and dispensing the liquid;

a first drive element engaged with or capable of being engaged with a first drive for displacing the movement element during at least one of aspirating and dispensing the liquid;

a liquid line into which the cavity discharges;

a pipette tip or an adapter for receiving a disposable pipette tip, the liquid line changing over to the pipette tip or the adaptor or including the pipette tip or the adapter;

a flush line, the hollow body including a flush inlet which discharges from the flush line into the cavity;

a carrier unit for moving the movement unit which is movably attached to the carrier unit in the direction of at least one of an X, or a Y, or a Z spatial axis, the carrier unit including at least one second drive element which is engaged with a second drive; and

wherein the movement unit comprises the hollow body and the movement element, the first drive element, the second drive element, and the pipette tip or the adapter, as well as a pulse generator for generating pressure waves the liquid in the cavity during dispensing, all these elements being movable together in at least one direction of the X, or the Y, or the Z spatial axis with respect to the carrier unit.

2. The device of claim 1, wherein the second drive element is implemented as a toothed rack on the outer surface of the hollow body.

3. The device of claim 1, wherein the hollow body, because of movability in at least one direction of the X, or the Y, or the Z spatial axis with respect to the carrier unit, is implemented for connecting and disconnecting the flush line and flush inlet as a slide valve in the region of the flush inlet or includes an additional valve there for opening and closing the flush inlet.

4. The device of claim 1, including sealing means positioned between a surface of the hollow body and a surface of the carrier unit.

5. The device of claim 1, wherein the flush inlet includes a contact point which is implemented so that an endpiece of the flush line is applied to the contact point using a movement of the hollow body in at least one direction of the X, or the Y, or the Z spatial axis to connect and disconnect the flush line and flush inlet.

6. The device of claim 1, wherein the movement element for at least one of aspirating and dispensing liquid is implemented as a piston and the cavity is implemented as a cylinder, the rear end of the piston being connected to a piezostack which is implemented as the pulse generator for emitting pulses onto the piston and therefore onto the liquids in the cavity, and the rear end of the piezostack is connected to the first drive element for raising and lowering the piston.

7. The device of claim 6, including a controller for moving the piston using the first drive or for moving the movement unit using the second drive, or for moving both drives for synchronizing the drives and for controlling the pulses of the piezostack.

8. The device of claim 1, wherein the movement element includes a membrane.

9. The device of claim 8, wherein the membrane is a passive membrane which is connected around its circumference to the inner wall of the hollow body and, on a rear side of the membrane, the membrane is connected to a piezostack which is implemented as the pulse generator for emitting pulses onto the passive membrane and therefore onto the liquid in the cavity, a rear end of the piezostack being connected to the first drive for raising and lowering the membrane.

10. The device of claim 9, wherein the first drive includes a DC motor having an encoder or a stepping motor, which acts on a wedge attached to the rear end of the piezostack via a transfer part connected to the motor and a miniature bearing attached to the transfer part.

11. The device of claim 10, including a controller for moving the membrane using the first drive or for moving the movement unit using the second drive, or for moving both drives, and pulses of the piezostack.

12. The device of claim 8, wherein the membrane is an active, bimorphous piezo membrane which is implemented as the pulse generator for emitting pulses onto the liquid in the cavity.

13. The device of claim 12, wherein the rear side or the circumference of the membrane is connected to a hollow cylinder which supports a wedge.

14. The device of claim 13, including a ring seal which seals the cavity between the circumference of the active membrane and the inner wall of the hollow body.

15. The device of claim 13, wherein the first drive includes a DC motor having an encoder or a stepping motor, which acts on the wedge attached to the hollow cylinder via a transfer part connected to the motor and a miniature bearing attached thereto.

16. The device of claim 15, including a controller for moving the wedge using the first drive or for moving the movement unit using the second drive, or for moving both drives, and pulses of the piezo membrane.

17. A system for at least one of aspirating and dispensing samples of a liquid, wherein the system includes one or more devices according to claim 1.

18. The system of claim 17, including a robot for moving the devices in at least one of the X, or the Y, or the Z direction.

19. The system of claim 17, including first drives and second drives having shafts which are implemented to engage, via switch elements, on the first drive element or on the second drive element, or on both drive elements, of the individual devices, or of linear groups, or of two-dimensional arrays of the devices.

20. The system of claim 19, wherein the linear the groups include 4, 8, 12, 16, 24, or 32 devices, and wherein the arrays include 16, 32, 96, 384, or 1536 devices.

22. A method of using a device according to claim 1, comprising pipetting or dispensing samples of a liquid.

23. The method of claim 22, wherein samples of a liquid are pipetted or dispensed in wells of a microplate.

24. The method of claim 22, wherein samples of a liquid are pipetted or dispensed on the surface of a target for sample investigation, particularly a MALDI TOF-MS target or a biochip.

21. The system of 17, including switches for linear displacement of selector shafts, which, depending on position, are engagable with the shafts of the drives or with the drive elements, or which are engagable with the shafts of the drives and with the drive elements.