Method of using G-matrix Fourier transformation nuclear magnetic resonance (GFT NMR) spectroscopy for rapid chemical shift assignment and secondary structure determination of proteins

  • US 6,831,459 B2
  • Filed: 07/11/2003
  • Issued: 12/14/2004
  • Est. Priority Date: 07/11/2002
  • Status: Active Grant
First Claim
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1. A method of conducting a (N,N−

  • K) dimensional (D) G-matrix Fourier transformation (GFT) nuclear magnetic resonance (NMR) experiment, wherein N is the dimensionality of an N-dimensional (ND) Fourier transformation (FT) NMR experiment and K is the desired reduction in dimensionality relative to N, said method comprising;

    providing a sample;

    applying radiofrequency pulses for the ND FT NMR experiment to the sample;

    selecting m indirect chemical shift evolution periods of the ND FT NMR experiment, wherein m equals K+1;

    jointly sampling the m indirect chemical shift evolution periods;

    independently cosine and sine modulating NMR signals detected in a direct dimension to generate (N−

    K)D basic NMR spectra comprising frequency domain signals with 2K chemical shift multiplet components, thereby enabling phase-sensitive sampling of all jointly sampled m indirect chemical shift evolution periods; and

    transforming the (N−

    K) D basic NMR spectra into (N−

    K) D phase-sensitively edited basic NMR spectra, wherein the 2K chemical shift multiplet components of the (N−

    K) D basic NMR spectra are edited to yield (N−

    K) D phase-sensitively edited basic NMR spectra having individual chemical shift multiplet components.

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