Optical-chemical sensor

• US 6,835,351 B2
• Filed: 01/02/2003
• Issued: 12/28/2004
• Est. Priority Date: 01/12/1999
• Status: Expired due to Term

• Current Assignee: AVL List GmbH (List Privatstiftung), Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Original Assignee: F Hoffmann-La Roche AG (Roche Holding AG)
• Inventors: Huber, Christian, Werner, Tobias, Young, Susannah, Bell, Douglas E., Wolfbeis, Otto S.
• Primary Examiner(s): Gakh, Yelens
• Assistant Examiner(s): SIEFKE, SAMUEL P
• Application Number:
  US10/335,843
• Publication Number:
  US 20030101803A1
• Time in Patent Office: 726 Days
• Field of Search: 422/82.07, 422/82.11, 436/125
• US Class Current: 422/82.07
• CPC Class Codes: G01N 2021/6432 Quenching
• CPC Class Codes: G01N 21/643 non-biological material
• CPC Class Codes: G01N 21/77 by observing the effect on a chemical indicator
• CPC Class Codes: G01N 2800/04 Endocrine or metabolic disorders
• CPC Class Codes: G01N 33/84 involving inorganic compounds or pH
• CPC Class Codes: Y10T 436/19 Halogen containing
• CPC Class Codes: Y10T 436/193333 In aqueous solution

Abstract

An optical-chemical sensor which is suitable for the continuous and discontinuous determination by luminescence optics of the concentration of chloride in an aqueous sample and which comprises a luminescence indicator (I) and a polymer (H) carrying the luminescence indicator (I) is characterized in that the luminescence indicator (I) is a non-lipophile acridine or bisacridine compound and the polymer (H) is a linear-chain hydrophile polymer soluble in an organic solvent, whereby it is possible to excite the sensor by commercially available LEDs, to manufacture very large numbers thereof at a moderate cost and in a reproducible way and, preferably, to use it for the determination of physiological chloride concentrations and the sensor furthermore has a wide dynamic measuring range for the determination of chloride; a high sensitivity, stability and reproducibility; a high selectivity for chloride; and a low pH cross-sensitivity.

Claims

1. An optical-chemical sensor which is suitable for the continuous and discontinuous determination by luminescence optics of the concentration of chloride in an aqueous sample and which comprises a luminescence indicator (I) and a polymer (H) carrying the luminescence indicator (I), characterized in that the luminescence indicator (I) is a non-lipophile acridine or bisacridine compound and the polymer (H) is a linear-chain hydrophile polymer soluble in an organic solvent, the luminescence indicator (I) being immobilized in the polymer (H).

2. An optical-chemical sensor according to claim 1, characterized in that the acridine or bisacridine compound is selected from a group comprising methylacridinium methosulfate (MAC), 4-nitrophenylbutylacridinium methosulfate (NPBA), N,N′

-di-(3-sulfopropyl)-9,9-bisacridinium (SPBA), N,N′

-diacetic acid ethyl ester-9,9-bisacridinium (AEBA) and lucigenine.

3. An optical-chemical sensor according to claim 1 or 2, characterized in that the linear-chain hydrophile polymer is a multiple block copolymer containing acid amide and/or acid imide and/or carboxylate and/or nitrile groups.

4. An optical-chemical sensor according to claim 1 or 2, characterized in that the polymer (H) carrying the luminescence indicator (I) is applied in the form of a film to a transparent carrier material (T).

5. An optical-chemical sensor according to claim 1 or 2, characterized in that the polymer (H) carrying the luminescence indicator (I) is embedded in the form of fine particles in a hydrogel film (G) applied to a transparent carrier material (T).

6. An optical-chemical sensor according to claim 4, characterized in that an additional layer (O) is applied to the film or hydrogel film (G) that is on the transparent carrier material (T), which layer is composed of a hydrophile ion-permeable polymer.

7. An optical-chemical sensor according to claim 4, characterized in that the transparent carrier material (T) is a light-conducting fiber.

8. An optical-chemical sensor according to claim 6 wherein the additional layer (O) contains at least one color pigment.

9. An optical-chemical sensor according to claim 6 wherein the additional layer (O) contains black pigment.

10. An optical-chemical sensor according to claim 3, characterized in that the polymer (H) carrying the luminescence indicator (I) is applied in the form of a film to a transparent carrier material (T).

11. An optical-chemical sensor according to claim 3, characterized in that the polymer (H) carrying the luminescence indicator (I) is embedded in the form of a fine particles in a hydrogel film (G) applied to a transparent carrier material (T).

12. An optical-chemical sensor according to claim 5, characterized in that an additional layer (O) is applied to the film or hydrogel film (G) that is on the transparent carrier material (T), which layer is composed of a hydrophile ion-permeable polymer.

13. An optical-chemical sensor according to claim 5, characterized in that the transparent carrier material (T) is a light-conducting fiber.

14. An optical-chemical sensor according to claim 6, characterized in that the transparent carrier material (T) is a light-conducting fiber.

15. An optical-chemical sensor according to claim 10, characterized in that the transparent carrier material (T) is a light-conducting fiber.

16. An optical-chemical sensor according to claim 11, characterized in that the transparent carrier material (T) is a light-conducting fiber.

17. An optical-chemical sensor according to claim 12, characterized in that the transparent carrier material (T) is a light-conducting fiber.

18. An optical-chemical sensor according to claim 11 wherein the additional layer (O) contains at least one color pigment.

19. An optical-chemical sensor according to claim 11 wherein the additional layer (O) contains black pigment.

20. An optical-chemical sensor according to claim 12 wherein the additional layer (O) contains at least one color pigment.

21. An optical-chemical sensor according to claim 12 wherein the additional layer (O) contains black pigment.