DNA isolation method
First Claim
1. A method of isolating DNA and/or cell nuclei in a microfabricated apparatus that contains a plurality of radially dispersed micro-channels the method comprising forming a mesh from a cell sample in one or more of the micro-channels which mesh acts as a barrier to DNA and/or cell nuclei.
3 Assignments
0 Petitions
Accused Products
Abstract
Disclosed is a method and apparatus for the isolation of DNA or cell nuclei or a mixture thereof from cell samples in a CD device. The method includes treating a suspension of whole cells with a lysis reagent so as to lyse the cytoplasmic membrane and at least some of the nuclear membranes, and introducing the lysate into micro-channels of a microfabricated apparatus in which each of the micro-channels is provided with a barrier disposed in the channel to impede the passage or flow of DNA and cell nuclei each while allowing the passage of liquid through the micro-channel so that a mesh comprising DNA is formed in the channel.
117 Citations
16 Claims
- 1. A method of isolating DNA and/or cell nuclei in a microfabricated apparatus that contains a plurality of radially dispersed micro-channels the method comprising forming a mesh from a cell sample in one or more of the micro-channels which mesh acts as a barrier to DNA and/or cell nuclei.
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3. A method of isolating DNA or cell nuclei or a mixture thereof from cells, which method comprises:
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a) treating a suspension of whole cells with a lysis reagent so as to lyse the cytoplasmic membranes and at least some of the nuclear membranes;
b) introducing the lysate from step a) into micro-channels of a microfabricated apparatus wherein each of said micro-channels incorporates means to impede the passage or flow of DNA and cell nuclei while allowing the passage of liquid through the micro-channel whereby a mesh comprising DNA is formed in the channel; and
,c) washing the mesh comprising DNA. - View Dependent Claims (4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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- 15. A microfabricated apparatus for isolating DNA or cell nuclei or a mixture thereof from cells, which apparatus comprises a rotatable disc, the disc comprising a sample introduction port located towards the center of the disc in contact with an annular sample reservoir which in turn is connected to a plurality of radially dispersed micro-channels, each micro-channel comprising an inlet channel and an outlet channel disposed towards the circumferential edge of the disc and a barrier disposed in each of the micro-channels to impede the passage or flow of DNA and cell nuclei, while allowing the passage of liquid there through, said barrier being selected from beads or raised structures.
Specification