Polymer matrix containing catalase co-immobilized with analytic enzyme that generates hydrogen peroxide

• US 6,858,403 B2
• Filed: 04/02/2001
• Issued: 02/22/2005
• Est. Priority Date: 05/11/1999
• Status: Expired due to Fees

• Current Assignee: M-Biotech, Inc.
• Original Assignee: M-Biotech, Inc.
• Inventors: Han, In Suk, Jung, Dal-Young
• Primary Examiner(s): Naff, David M.
• Application Number:
  US09/824,552
• Publication Number:
  US 20020006634A1
• Time in Patent Office: 1,422 Days
• Field of Search: 435/174, 435/176, 435/180, 435/182, 435/817, 435/14, 435/27, 435/175, 435/287.1
• US Class Current: 435/27
• CPC Class Codes: A61B 5/0002 Remote monitoring of patients using telemetry, e.g. transmission of vital signals via a communication network A61B5/07 takes precedence; transmission systems for measured values G08C; transmission H04B; transmission of digital information H04L; wireless communication networks H04W
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: C12N 11/18 Multi-enzyme systems
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: G01N 13/04 Investigating osmotic effects
• CPC Class Codes: G01N 7/00 Analysing materials by measuring the pressure or volume of a gas or vapour
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

Hydrogels containing catalase co-immobilized with an analyte-sensitive enzyme such as glucose oxidase are disclosed. The hydrogels may be pH-sensitive, and preferably are thin and lightly crosslinked. The catalase is present in concentrations ranging generally from 100 units/ml to about 1000 units/ml. These hydrogels have much faster swelling response times as compared to hydrogels without catalase, and are useful in biosensors and analyte-responsive drug delivery devices. The hydrogels also have an increased useful life, due to protection of the immobilize analyte-sensitive enzyme from degradation by hydrogen peroxide.

Claims

1. A polymer matrix incorporating catalase co-immobilized with an analytic enzyme which generates hydrogen peroxide, wherein the concentration of the catalase in a pregel solution which gels to form the polymer matrix ranges from about 100 units/mL to about 1000 units/mL.

2. The polymer matrix of claim 1, which is pH-sensitive.

3. The polymer matrix of claim 1, which consists of between about 0.5 mol % and about 6 mol % cross-linking monomers.

4. The polymer matrix of claim 1, which when hydrated has a thickness ranging from about 0.1 mm to about 3.0 mm.

5. The polymer matrix of claim 1, wherein the analytic enzyme is glucose oxidase.

6. The polymer matrix of claim 1, wherein the matrix is composed of hydroxypropyl methacrylate, N,N-dimethylaminoethyl methacrylate, and tetraethyleneneglycol dimethacrylate.

7. The polymer matrix of claim 1 in combination with a biosensor in which the polymer matrix is contained, the biosensor including means to monitor and detect changes in the polymer matrix.

8. The polymer matrix-biosensor combination of claim 7, wherein the biosensor is an analyte-responsive drug delivery device, wherein the polymer matrix changes in response to an analyte condition, and wherein the changes in the polymer matrix control the drug delivery from the device.

9. A method of making a polymer matrix containing catalase co-immoblized with an analytic enzyme which generates hydrogen peroxide for use in a biosensor or analyte-responsive drug delivery device, comprising the steps of:

making a pregel solution containing an added analytic enzyme which generates hydrogen peroxide and catalase where the catalase is added at a concentration ranging from about 100 units of catalase/mL pregel solution to about 1000 units catalase/mL pregel solution; and

polymerizing the pregel solution to form a polymer matrix and co-immobilize the analytic enzyme with the catalase in the polymer matrix.

10. The method of claim 9, wherein the polymer matrix has between about 0.5 mol % and about 6 mol % cross-linking monomers.

11. The method of claim 10, wherein the polymer matrix is formed to have a thickness when hydrated of between about 0.1 mm and about 3.0 mm.

12. The method of claim 10, wherein the analytic enzyme is glucose oxidase.