Process for delivering nucleic acids to cardiac tissue
First Claim
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1. A process for delivering nucleic acids to muscle cells in a mammalian heart, comprising:
- a) accessing an in vivo blood vessel; and
, b) inserting an injector into the blood vessel near or in the heart, in vivo, and injecting a solution containing nucleic acids into the blood vessel lumen and increasing hydrostatic pressure in the lumen thereby delivering the nucleic acids to the heart muscle cells outside of the blood vessel via the pressure, wherein invasiveness is limited to accessing the blood vessel, inserting the injector into the blood vessel, and injecting the solution.
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Abstract
A process for delivering a nucleic acid to a cardiac tissue cell in a mammal is described, comprising introducing a composition consisting of a nucleic acid to a blood vessel, which subsequently delivers the nucleic acid to the cardiac tissue cell. The nucleic acid can be DNA or RNA or plasmid DNA or viral. This process is for purposes of gene therapy, and research.
30 Citations
10 Claims
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1. A process for delivering nucleic acids to muscle cells in a mammalian heart, comprising:
- a) accessing an in vivo blood vessel; and
, b) inserting an injector into the blood vessel near or in the heart, in vivo, and injecting a solution containing nucleic acids into the blood vessel lumen and increasing hydrostatic pressure in the lumen thereby delivering the nucleic acids to the heart muscle cells outside of the blood vessel via the pressure, wherein invasiveness is limited to accessing the blood vessel, inserting the injector into the blood vessel, and injecting the solution. - View Dependent Claims (2, 3, 4, 5, 6)
- a) accessing an in vivo blood vessel; and
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7. A process for gene expression in mammalian heart muscle cells, comprising:
- a) accessing an in vivo blood vessel;
b) inserting an injector into the blood vessel near or in the heart, in vivo, and injecting a solution containing nucleic acids encoding a protein into the blood vessel lumen and increasing hydrostatic pressure in the lumen thereby delivering the nucleic acids to the heart muscle cells outside of the blood vessel via the pressure; and
, expressing the nucleic acids to provide a therapeutic protein, wherein invasiveness is limited to accessing the blood vessel, inserting the injector into the blood vessel, and injecting the solution. - View Dependent Claims (8, 9, 10)
- a) accessing an in vivo blood vessel;
Specification
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Current AssigneeRoche Madison Inc. (Arrowhead Pharmaceuticals, Inc.), Wisconsin Alumni Research Foundation (University of Wisconsin System)
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Original AssigneeMirus Bio LLC
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InventorsWolff, Matthew R., Whitesell, Larry F., Rozema, David B., Monahan, Sean D., Herweijer, Hans, Hagstrom, James E., Wolff, Jon A., Budker, Vladimir G., Slattum, Paul M.
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Primary Examiner(s)WOITACH, JOSEPH T
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Application NumberUS09/330,909Time in Patent Office2,104 DaysField of Search424/93.21, 514/44, 435/115US Class Current514/44.00RCPC Class CodesA61K 48/0008 characterised by an aspect ...A61K 48/0075 characterised by an aspect ...
