Biosensor

• US 6,916,410 B2
• Filed: 05/15/2002
• Issued: 07/12/2005
• Est. Priority Date: 11/15/1999
• Status: Expired due to Term

• Current Assignee: ARKRAY, Inc
• Original Assignee: ARKRAY, Inc
• Inventors: Katsuki, Koji, Fukuoka, Takao, Hamamoto, Katsumi, Yagi, Yuji
• Primary Examiner(s): Noguerola, Alex
• Application Number:
  US10/147,213
• Publication Number:
  US 20020189941A1
• Time in Patent Office: 1,154 Days
• Field of Search: 204/403.01, 204/403.06, 204/403.1, 204/403.11, 204/403.14, 204/409
• US Class Current: 204/403.05
• CPC Class Codes: C12Q 1/001 Enzyme electrodes

Abstract

A biosensor is constituted by disposing an electrode system including a working electrode and a counter electrode on a substrate, forming an inorganic particulate-containing layer containing inorganic particulates thereon, and forming a reagent layer containing a reagent thereon. The inorganic particulates make it possible to prevent impurities in a sample from being in contact with the electrode system and being adsorbed therein, so that measurement is performed with high precision. The inorganic particulate-containing layer can be formed by applying a dispersion system of inorganic particulates and drying them, and it is preferable that the inorganic particulates are contained in the form of aggregates.

Claims

1. A biosensor comprising a substrate, a reagent layer containing a reagent, and an electrode system including a working electrode and a counter electrode, the electrode system being disposed on the substrate, and the reagent layer being formed on the electrode system,

wherein the reagent layer further comprises expansive inorganic particulates and is a laminate comprising a reagent-containing layer containing the reagent and a particulate-containing layer containing the expansive inorganic particulates.

2. The biosensor according to claim 1, wherein the expansive inorganic particulate-containing layer is present between the reagent-containing layer and the electrode system.

3. The biosensor according to claim 1, wherein the reagent layer contains aggregates of expansive inorganic particulates.

4. The biosensor according to claim 1, wherein a layer containing expansive inorganic particulates is formed by applying a dispersion solution in which the expansive inorganic particulates are dispersed on the electrodes, and drying the dispersion solution.

5. The biosensor according to claim 1, wherein the expansive inorganic particulates are particulates of clay mineral.

6. The biosensor according to claim 5, wherein the clay mineral is expansive phyllosilicate.

7. The biosensor according to claim 6, wherein the expansive phyllosilicate is at least one of smectite and expansive mica.

8. The biosensor according to claim 7, wherein the smectite is at least one selected from the group consisting of hectorite, saponite, and montmorillonite.

9. The biosensor according to claim 7, wherein the expansive mica is at least one of sodium tetrasilicon fluoride mica and teniorite.

10. The biosensor according to claim 1, wherein the content of the expansive inorganic particulates in the layer containing expansive inorganic particulates is in a range from 0.14 to 14 mg per cm² of an area.

11. The biosensor according to claim 1, wherein the thickness of the layer containing expansive inorganic particulates is in a range from 0.05 to 5 μ

m.

12. The biosensor according to claim 1, wherein the reagent layer further comprises particulates of a water-insoluble high molecular weight compound.

13. The biosensor according to claim 12, wherein the water-insoluble high molecular weight compound is a polymer or a copolymer containing at least one of acrylic acid, methacrylic acid, maleic acid, acrylic ester, methacrylic acid ester, maleic acid ester, styrene, and styrene derivative monomer, or a polyamide-based high molecular weight compound.

14. The biosensor according to claim 12, wherein the average particle diameter of the particulates of the water-insoluble high molecular weight compound is in a range from 0.1 to 45 μ

m.

15. The biosensor according to claim 1, further comprising a layer containing particulates of a water-insoluble high molecular weight compound, the reagent layer being formed between the electrode system and the layer containing particulates of a water-insoluble high molecular weight compound.

16. The biosensor according to claim 1, wherein a surfactant-containing layer containing a surfactant further is formed on the reagent layer.

17. The biosensor according to claim 1, wherein the electrodes are at least one selected from the group consisting of gold electrodes, carbon electrodes and silver electrodes.

18. The biosensor according to claim 1, wherein the reagent contains a redox enzyme.

19. The biosensor according to claim 1, wherein the reagent contains a redox enzyme and an electron acceptor in a reaction of the enzyme.

20. The biosensor according to claim 1, wherein the sample to be measured is blood.