Hydrogel-based microarray signal amplification methods and devices therefor
First Claim
Patent Images
1. A method of detecting a target nucleic acid or protein, comprising:
- (a) providing a target comprising a target nucleic acid or protein and a first member of a binding pair;
(b) hybridizing the target nucleic acid or protein to a probe attached to a hydrogel matrix through a 2+2 photocycloaddition;
(c) contacting the first member of the binding pair with a second member of the binding pair, said second member comprising a fluorophore; and
(d) detecting the fluorophore additions, thereby detecting said target nucleic acid or protein.
3 Assignments
0 Petitions
Accused Products
Abstract
Methods and devices for detecting nucleic acid and protein targets on hydrogel microarrays are disclosed. Fluorophores are incorporated into the targets and detected. A linear correlation between target concentration and signal amplitude is maintained through the elimination of active enzyme amplification.
23 Citations
38 Claims
-
1. A method of detecting a target nucleic acid or protein, comprising:
-
(a) providing a target comprising a target nucleic acid or protein and a first member of a binding pair;
(b) hybridizing the target nucleic acid or protein to a probe attached to a hydrogel matrix through a 2+2 photocycloaddition;
(c) contacting the first member of the binding pair with a second member of the binding pair, said second member comprising a fluorophore; and
(d) detecting the fluorophore additions, thereby detecting said target nucleic acid or protein. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 30)
-
-
23. A method of detecting a single nucleotide polymorphism, comprising:
-
(a) hybridizing a target nucleic acid to a probe attached to a hydrogel matrix through a 2+2 photocycloaddition, said probe designed to terminate at the site of the single nucleotide polymorphism;
(b) extending the probe by one nucleotide, wherein the nucleotide comprises a first member of a binding pair;
(c) contacting the first member of the binding pair with a second member of the binding pair comprising a fluorophore; and
(d) detecting the fluorophore additions, thereby detecting the single nucleotide polymorphism. - View Dependent Claims (24, 25, 26, 27, 28, 29, 31, 32, 33, 34, 35, 36, 37, 38)
-
Specification