Lentiviral LTR-deleted vector

• US 6,924,123 B2
• Filed: 12/20/2002
• Issued: 08/02/2005
• Est. Priority Date: 10/29/1996
• Status: Expired due to Term

• Current Assignee: Oxford Biomedica (UK) Limited
• Original Assignee: Oxford Biomedica (UK) Limited
• Inventors: Kingsman, Susan Mary, Kingsman, Alan John
• Primary Examiner(s): Guzo, David
• Application Number:
  US10/324,616
• Publication Number:
  US 20030113920A1
• Time in Patent Office: 956 Days
• Field of Search: 424/93.1, 424/93.2, 424/93.6, 435/235.1, 435/320.1, 435/69.1, 435/325, 435/366
• US Class Current: 435/69.1
• CPC Class Codes: A61K 48/00 Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: C12N 15/62 DNA sequences coding for fusion proteins
• CPC Class Codes: C12N 15/86 Viral vectors
• CPC Class Codes: C12N 2740/15043 viral genome or elements thereof as genetic vector
• CPC Class Codes: C12N 2740/16043 viral genome or elements thereof as genetic vector
• CPC Class Codes: C12N 2799/027 where the vector is derived from a retrovirus
• CPC Class Codes: C12N 2830/00 Vector systems having a special element relevant for transcription
• CPC Class Codes: C12N 2830/15 chimeric enhancer/promoter combination
• CPC Class Codes: C12N 2830/48 regulating transport or export of RNA, e.g. RRE, PRE, WPRE, CTE
• CPC Class Codes: C12N 2830/50 regulating RNA stability, not being an intron, e.g. poly A signal
• CPC Class Codes: C12N 2830/60 from viruses
• CPC Class Codes: C12N 9/0071 acting on paired donors with incorporation of molecular oxygen (1.14)
• CPC Class Codes: C12N 9/88 Lyases (4.)

Abstract

A vector capable of transducing non-dividing and/or slowly dividing cells is provided, wherein the vector is a lentiviral LTR-deleted vector. Also provided is a method for producing a protein of interest in a non-dividing or slowly dividing cell by transducing the cell with a lentiviral LTR-deleted vector and expressing the protein of interest in the cell. In addition, target cells containing the lentiviral LTR-deleted vector are provided.

Claims

1. A lentiviral vector particle capable of transducing a non-dividing or slowly-dividing cell, said vector particle comprising a lentiviral LTR-deleted (LCD) vector, wherein the LLD vector comprises sequences sufficient for reverse transcription and integration, and wherein, upon transduction, the LLD vector comprises a non-functional lentiviral 5′

LTR promoter.

2. The vector particle according to claim 1, further comprising a nucleotide sequence of interest (NOI).

3. A method for producing a protein of interest in a non-dividing or slowly-dividing cell, comprising the steps of:

a) transducing the cell with the vector particle according to claim 2, wherein the NOI encodes a protein of interest; and

b) expressing the protein of interest in the cell.

4. The method according to claim 3, wherein the non-dividing cell is a neuron.

5. A target cell in vitro comprising the vector particle of claim 1.

6. A target cell in vitro comprising the vector particle of claim 2.