Mesoscale polynucleotide amplification device and method

  • US 6,953,676 B1
  • Filed: 12/15/1998
  • Issued: 10/11/2005
  • Est. Priority Date: 05/01/1992
  • Status: Expired due to Fees
First Claim
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1. A device for amplifying a preselected polynucleotide in a sample by conducting a polynucleotide polymerization reaction, the device comprising:

  • a solid substrate microfabricated to define;

    a sample inlet port; and

    a mesoscale flow system comprising;

    a sample flow channel having a cross-section and extending from said inlet port; and

    a polynucleotide polymerization reaction chamber having a cross-section and being in fluid communication with said flow channel, said chamber containing reagents for the polymerization reaction, said flow channel and said reaction chamber having at least one cross-sectional dimension of width or depth which is between about 0.1 to 500 μ

    m said reaction chamber cross-section and said flow channel cross-section being dissimilar;

    means for thermally regulating the contents of said chamber whereby the temperature is controlled to amplify said preselected polynucleotide; and

    means for detecting said amplified polynucleotide.

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