Nanocrystals

• US 6,955,855 B2
• Filed: 09/17/2002
• Issued: 10/18/2005
• Est. Priority Date: 09/17/2001
• Status: Expired due to Term

• Current Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Original Assignee: BioCrystal, Ltd.
• Inventors: Naasani, Imad
• Primary Examiner(s): Kiliman, Leszek
• Application Number:
  US10/245,082
• Publication Number:
  US 20030059635A1
• Time in Patent Office: 1,127 Days
• Field of Search: 428/403, 428/407, 428/690, 252/301.4 R, 252/301.4 S, 252/301.6 R, 252/301.6 S
• US Class Current: 428/403
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: G01N 33/588 with semiconductor nanocrystal label, e.g. quantum dots
• CPC Class Codes: Y10T 428/25 Web or sheet containing structurally defined element or component and including a second component containing structurally defined particles
• CPC Class Codes: Y10T 428/2991 Coated
• CPC Class Codes: Y10T 428/2993 Silicic or refractory material containing [e.g., tungsten oxide, glass, cement, etc.]
• CPC Class Codes: Y10T 428/2998 including synthetic resin or polymer
• CPC Class Codes: Y10T 428/31721 Of polyimide

Abstract

Provided herein are compositions of functionalized, fluorescent nanocrystals comprising fluorescent nanocrystals coated with an imidazole-containing compound; compositions of functionalized, fluorescent nanocrystals comprising fluorescent nanocrystals coated with an imidazole-containing compound and cross-linked with a phosphine cross-linking compound; compositions of functionalized fluorescent nanocrystals operably bound to molecular probe; a process of making functionalized, fluorescent nanocrystals; and a process of using functionalized, fluorescent nanocrystals in a detection system.

Claims

1. A fluorescent nanocrystal composition comprising:

a fluorescent nanocrystal; and

a coating over said fluorescent nanocrystal, wherein said coating comprises an imidazole-containing compound operably bound by an imidazole group to a metal cation.

2. The fluorescent nanocrystal composition of claim 1, wherein said metal cation is operably bound to a sulfur containing compound.

3. The fluorescent nanocrystal composition of claim 1, wherein said metal cation comprises semiconductor material.

4. The fluorescent nanocrystal composition of claim 1, further comprising a molecular probe, one or more molecules of which are operably bound to the coated fluorescent nanocrystal.

5. The fluorescent nanocrystal composition of claim 1, wherein said fluorescent nanocrystal is selected from the group of core semiconductor nanocrystals, core/shell semiconductor nanocrystals, doped metal oxide nanocrystals, and combinations thereof.

6. The fluorescent nanocrystal composition as set forth in claim 1, wherein said fluorescent nanocrystal composition fluoresces with at least about 10 fold more intensity than the fluorescent nanocrystal, said fluorescent nanocrystal composition and fluorescent nanocrystals excited with an excitation source suitable to elicit fluorescence from the coated fluorescent nanocrystal.

7. The fluorescent nanocrystal composition as set forth in claim 1, further comprising a cross-linking agent.

8. The fluorescent nanocrystal composition as set forth in claim 7, wherein said cross-linking agent is selected from the group consisting of an amino-reactive agent, carboxyl-reactive agent, and a combination including amino-reactive and carboxyl-reactive agents.

9. The fluorescent nanocrystal composition as set forth in claim 7, wherein said cross-linking agent comprises a phosphine cross-linking compound.

10. A fluorescent nanocrystal composition comprising:

a fluorescent nanocrystal; and

a coating over said fluorescent nanocrystal, wherein said coating comprises an imidazole-containing compound, said imidazole-containing compound operably bound by an imidazole group to said fluorescent nanocrystal.

11. The fluorescent nanocrystal composition as set forth in claim 10, further including a cross linking agent.

12. The fluorescent nanocrystal composition as set forth in claim 10, wherein said cross-linking agent is selected from the group consisting of an amino-reactive agent, carboxyl-reactive agent, and a combination including amino-reactive and carboxyl-reactive agents.

13. The fluorescent nanocrystal composition as set forth in claim 10, wherein said cross-linking agent comprises a phosphine cross-linking compound.

14. The fluorescent nanocrystal composition as set forth in claim 10 further comprising a molecular probe, one or more molecules of which are operably bound to the coated fluorescent nanocrystal.

15. The nanocrystal as set forth in claim 11, wherein said imidazole-containing compound operably bound by an imidazole group to said fluorescent nanocrystal is a dipeptide.

16. The fluorescent nanocrystal composition as set forth in claim 11, wherein said fluorescent nanocrystal composition fluoresces with at least about 10 fold more intensity than the fluorescent nanocrystal, said fluorescent nanocrystal composition and fluorescent nanocrystals excited with an excitation source suitable to elicit fluorescence from the coated fluorescent nanocrystal.

17. The fluorescent nanocrystal composition as set forth in claim 10, wherein the fluorescent nanocrystal is selected from the group of core semiconductor nanocrystals, core/shell semiconductor nanocrystals, doped metal oxide nanocrystals, and combinations thereof.

18. The fluorescent nanocrystal composition as set forth in claim 15, further including a cross linking agent.

19. A process of making coated nanocrystals comprising;

contacting a solution comprising imidazole-containing compound with a metal cation solution to produce a mixture; and

contacting said mixture with fluorescent nanocrystals to form a coating over the fluorescent nanocrystals wherein said coating comprises an imidizole-containing compound operably bound by an imidizole group to a metal cation.

20. The process as set forth in claim 19, wherein said metal cation is operably bound to a sulfur containing compound.

21. The process as set forth in claim 19, wherein said metal cation comprises semiconductor material.

22. The process an set forth in claim 19, further comprising operably binding the coated fluorescent nanocrystal to a molecular probe.

23. The process as set forth in claim 19, wherein the fluorescent nanocrystal is selected from the group of core semiconductor nanocrystals, core/shell semiconductor nanocrystals, doped metal oxide nanocrystals, and combinations thereof.

24. A process of making coated nanocrystals comprising:

contacting a solution comprising imidazole-containing compound with fluorescent nanocrystals to form a coating over the fluorescent nanocrystals, said imidazole-containing compound operably bound by an imididazole group to said fluorescent nanocrystals.

25. The process of claim 24, further including the act of contacting said imidazole-containing compound operably bound by an imidazole group to said fluorescent nanocrystal with a cross-linking agent.

26. The process of claim 25, wherein said cross-linking agent comprises a phosphine cross-linking compound.

27. The process of claim 25, wherein said cross-linking agent is selected from the group consisting of an amino-reactive agent, carboxyl-reactive agent, or a combination of amino-reactive and carboxyl-reactive agents.

28. The process of claim 24, wherein said imidazole containing compound is a dipeptide.

29. The process of claim 24, further comprising operably binding the coated fluorescent nanocrystal to one or more molecules of a molecular probe.

30. The process of claim 24, wherein the fluorescent nanocrystal is selected from the group of core semiconductor nanocrystals, core/shell semiconductor nanocrystals, doped metal oxide nanocrystal, and combinations thereof.

31. A method of making water soluble fluorescent nanocrystals comprising:

operably bonding an imidazole group of an imidazole-containing compound with fluorescent nanocrystals.