Sensor

• US 6,972,423 B2
• Filed: 02/06/2003
• Issued: 12/06/2005
• Est. Priority Date: 01/21/1998
• Status: Expired due to Fees

• Current Assignee: Cambridge University (Government of United Kingdom)
• Original Assignee: Cambridge University (Government of United Kingdom)
• Inventors: Rayment, Trevor, Welland, Mark E.
• Primary Examiner(s): LE, QUE TAN
• Application Number:
  US10/360,513
• Publication Number:
  US 20030222232A1
• Time in Patent Office: 1,034 Days
• Field of Search: 250/573, 250/222.2, 250/574, 356/338, 356/436, 356/441, 435/6, 435/526, 436/518, 436/161, 436/524
• US Class Current: 250/573
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Abstract

The present invention pertains to a sensor, suitable for use in the detection of an analyte in a fluid, comprising: a tube (4) defining a flow path for the fluid; a generally planar member (3) mounted in the flow path (→) such that the plane is in the direction of flow, wherein the member has, bound thereto, a ligand that interacts with the analyte, wherein interaction causes the member to flex; and means (1, 2) for the detection of the flexing.

Claims

1. A sensor for use in the detection of an analyte in a fluid, the sensor comprising:

a tube defining a flow path for the fluid;

a generally planar member having opposed faces mounted in the flow path such that the plane is in the direction of flow, wherein the member has, bound thereto, in separate areas, a ligand and a reference compound, wherein the ligand interacts with the analyte, and wherein the interaction causes different stresses to act on the opposed faces of the member, thereby causing the member to flex;

means for the detection of the flexing; and

means for distinguishing between the ligand signal and the reference compound signal, whereby common mode noise can be determined.

2. The sensor according to claim 1, wherein the ligand is bound to one face of the member, and the other face is coated with a material that is responsive to the detection means.

3. The sensor according to claim 2, wherein the detection means comprises a laser.

4. The sensor according to claim 1, wherein the detection means comprises a laser.

5. The sensor according to claim 1, wherein the member includes an aperture, whereby its torsional rigidity is increased.

6. The sensor according to claim 1, which comprises a plurality of the members, positioned in series, having respective ligands bound thereto.

7. The sensor according to claim 1, wherein the fluid is a liquid.

8. A method for screening a target compound with respect to a plurality of analytes in a fluid, which comprises:

causing the analytes to flow through the tube of a sensor, wherein the sensor comprises a tube defining a flow path for the fluid;

a generally planar member having opposed faces mounted in the flow path such that the plane is in the direction of flow, wherein the member has, bound thereto, in separate areas, a ligand and a reference compound, the target compound being the ligand, wherein the ligand interacts with the analyte, and wherein the interaction causes different stresses to act on the opposed faces of the member, thereby causing the member to flex;

means for the detection of the flexing; and

means for distinguishing between the ligand signal and the reference compound signal, whereby common mode noise can be determined; and

detecting any flexing of the member.

9. The method according to claim 8, wherein the analytes are provided successively, in liquid form, and bubbles are introduced between each liquid analyte, thereby allowing calibration of the device and optionally also flushing liquid between analytes.

10. The method according to claim 8, wherein the fluid is a liquid.

11. The method according to claim 8, wherein the ligand is bound to one face of the member of the sensor, and the other face is coated with a material that is responsive to the detection means.

12. The method according to claim 8, wherein the detection means of the sensor comprises a laser.

13. The method according to claim 8, wherein the member of the sensor includes an aperture, whereby its torsional rigidity is increased.

14. The method according to claim 8, wherein the sensor comprises a plurality of the members, positioned in series, having respective ligands bound thereto.