Biosensor

• US 6,982,027 B2
• Filed: 10/25/2001
• Issued: 01/03/2006
• Est. Priority Date: 10/27/2000
• Status: Expired due to Term

• Current Assignee: ARKRAY, Inc
• Original Assignee: ARKRAY, Inc
• Inventors: Yagi, Yuji
• Primary Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US10/399,874
• Publication Number:
  US 20040040839A1
• Time in Patent Office: 1,531 Days
• Field of Search: 20440301-40314, 204416-418
• US Class Current: 204/403.06
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: C12Q 1/002 Electrode membranes

Abstract

A biosensor is provided, which can measure an analyte in a sample with high precision. The biosensor is produced by disposing a electrode system including a working electrode 12 and a counter electrode 13 on a substrate 11, and then forming a reagent layer 16 containing a reagent and particulates on the electrode system. The influence of the impurities in the sample on the electrode system can be eliminated by the particulates. The average particle diameter of the particulates preferably is in the range from 0.1 to 45 μm.

Claims

1. A biosensor comprising a substrate, a reagent layer containing a reagent, and an electrode system including a working electrode and a counter electrode, the electrode system being disposed on the substrate, and the reagent layer being formed on the electrode system,

wherein the reagent layer further comprises particulates, and

an inorganic gel-containing layer containing an inorganic gel is formed between the electrode system and the reagent layer.

2. The biosensor according to claim 1, wherein the reagent layer is a single layer.

3. The biosensor according to claim 1, wherein the reagent layer is a laminate comprising a reagent-containing layer containing the reagent and a particulate-containing layer containing the particulates.

4. The biosensor according to claim 3, wherein the particulate-containing layer is formed on the electrode system via the reagent-containing layer.

5. The biosensor according to claim 1, wherein the reagent layer further comprises an inorganic gel.

6. The biosensor according to claim 5, wherein the inorganic gel is a gel of clay mineral.

7. The biosensor according to claim 6, wherein the clay mineral is expansive phyllosilicate.

8. The biosensor according to claim 1, wherein a surfactant-containing layer containing a surfactant further is formed on the reagent layer.

9. The biosensor according to claim 1, wherein an average particle diameter of the particulates is in a range from 0.1 to 45 μ

m.

10. The biosensor according to claim 1, wherein particle size distribution of the particulates is in a range from 0.01 to 100 μ

m.

11. The biosensor according to claim 1, wherein the particulates are spherical.

12. The biosensor according to claim 1, wherein the particulates are formed of a polymer compound.

13. The biosensor according to claim 12, wherein the polymer compound does not contain an impurity causing electrolysis and is electrochemically inactive.

14. The biosensor according to claim 12, wherein the polymer compound is water-insoluble.

15. The biosensor according to claim 12, wherein the polymer compound is a polymer or a copolymer containing at least one of acrylic acid, methacrylate, maleate, acrylic ester, methacrylic acid ester, maleate ester, and styrene derivative monomer, or a polyamide-based polymer compound.

16. The biosensor according to claim 1, wherein the electrodes are at least one selected from the group consisting of gold electrodes, carbon electrodes, and silver electrodes.

17. The biosensor according to claim 1, wherein the reagent contains a redox enzyme.

18. The biosensor according to claim 1, wherein the reagent contains a redox enzyme and an electron acceptor in a reaction of the enzyme.

19. The biosensor according to claim 1, wherein a sample to be measured is blood.