Apparatus and method for assessing the liquid flow performances through a small dispensing orifice

• US 6,983,636 B2
• Filed: 01/27/2003
• Issued: 01/10/2006
• Est. Priority Date: 01/25/2002
• Status: Expired due to Term

• Current Assignee: BioNex Solutions, Inc.
• Original Assignee: Innovadyne Technologies, Inc.
• Inventors: Johnson, James E., Picha, Neil R., Storms, Craig M., Martin, David A.
• Primary Examiner(s): Noland, Thomas P.
• Application Number:
  US10/353,804
• Publication Number:
  US 20030167822A1
• Time in Patent Office: 1,079 Days
• Field of Search: 73/13.6, 73/17.4, 250/252.1, 250/573, 250/576
• US Class Current: 73/1.36
• CPC Class Codes: B01L 2200/143 Quality control, feedback systems
• CPC Class Codes: B01L 2200/148 Specific details about calibrations
• CPC Class Codes: B01L 2300/14 Means for pressure control
• CPC Class Codes: B01L 2400/02 Drop detachment mechanisms of single droplets from nozzles or pins
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/06 Valves, specific forms thereof
• CPC Class Codes: B01L 2400/0633 with moving parts
• CPC Class Codes: B01L 2400/0666 Solenoid valves
• CPC Class Codes: B01L 3/021 Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
• CPC Class Codes: B01L 3/0268 using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
• CPC Class Codes: G01N 2035/00237 Handling microquantities of analyte, e.g. microvalves, capillary networks
• CPC Class Codes: G01N 2035/1041 Ink-jet like dispensers
• CPC Class Codes: G01N 2035/1044 Using pneumatic means
• CPC Class Codes: G01N 35/00594 Quality control, including calibration or testing of components of the analyser
• CPC Class Codes: G01N 35/00712 Automatic status testing, e.g. at start-up or periodic
• CPC Class Codes: G01N 35/1002 Reagent dispensers
• CPC Class Codes: G01N 35/1016 Control of the volume dispensed or introduced
• CPC Class Codes: G01N 35/1065 Multiple transfer devices
• CPC Class Codes: Y10T 137/3003 Fluid separating traps or vents
• CPC Class Codes: Y10T 436/15 Inorganic acid or base [e.g. , hcl, sulfuric acid, etc. ]
• CPC Class Codes: Y10T 436/2575 : Volumetric liquid transfer

Abstract

A universal calibration apparatus and method to estimate the dispense output from a low volume, non-contact, liquid dispensing systems that may be applied for every hardware configuration (e.g., tube length, orifice diameter, tip design, etc), reagent solution property and environmental condition. This same calibration technique is applied to calibrate or tune these non-contact liquid dispensing systems to dispense desired volumes (in the range of about 0.050 μL to 50 μL), irrespective of the hardware configuration or the solution properties. That is, the calibration technique is not dependent on any variables, but the result (the actual dispense volume) is dependant on the variable mentioned. By actuating selected pulse widths, and measuring the resulting volume, a Calibration Profile can be generated correlating the liquid volume dispensed from the orifice to the respective pulse width of the dispensing valve thereof through calibration points. In particular, one is selected to deliver a first volume of liquid that is less than a lower base pulse width correlating to the lowest volume of the selected range of volumes of liquid, while a second pulse width is selected to deliver a second volume of liquid dispensed that is greater than an upper ceiling pulse width correlating to the highest volume of the selected range of volumes of liquid. Intermediary pulse widths are also applied, each selected to deliver a different, spaced-apart, respective intermediary low volumes of liquid dispensed from the dispensing orifice between the first volume and the second volume. Thus, applying the Calibration Profile, the pulse widths correlating to the one or more targeted discrete volumes for liquid dispensing can be extrapolated.

Claims

1. A method for assessing the liquid flow performance for dispensing liquid through a relatively small diameter dispensing orifice fluidly coupled to a communication passageway of a precision, low volume, liquid handling system, the method comprising:

emitting an optical beam of a sensor assembly, from a position outboard from one side of the dispensing orifice, along an optical path extending substantially laterally across and downstream from the dispensing orifice of the liquid handling system prior to dispensing liquid from the dispensing orifice;

continuously sensing the optical beam along the optical path, from a position outboard from an opposite side of the dispensing orifice;

flowing the dispensing liquid through the communication passageway generally sufficient to eject at least a drop of dispensing liquid from the dispensing orifice, and across the optical path of the optical beam; and

adjusting the sensitivity of the sensor assembly so that the drop of dispensed liquid from the dispensing orifice is more “

visible”

to the sensor assembly, wherein;

detecting the drop indicates a flow condition of the dispensing fluid through the dispensing orifice; and

wherein

not detecting the drop indicates a non-flow condition of the dispensing fluid through the dispensing orifice.

2. The method according to claim 1, wherein

the emitting of an optical beam includes activating a laser diode emitting the optical beam.

3. The method according to claim 1, wherein

the continuously sensing the optical beam includes detecting the optical beam through a receiving component of said sensor assembly configured to detect the optical beam.

4. The method according to claim 1, wherein

the adjusting the sensitivity of the sensor assembly includes adjusting the sensitivity of a receiving component of the sensor assembly so that the drop of dispensed liquid from the dispensing orifice is more “

visible”

to the receiving component.

5. The method according to claim 1, wherein

the detecting the drop includes identifying a change in the continuous sensing of the optical beam, and

not detecting the drop includes not identifying a change in the continuous sensing of the optical beam.

6. The method according to claim 5, wherein

the identifying a change includes detecting the pause in the continuous sensing of the optical beam, and

the not identifying a change includes not detecting a pause in the continuous sensing of the optical beam.

7. The method according to claim 1, wherein

the adjusting the sensitivity of the sensor assembly includes adjusting the intensity of the optical beam.

8. The method according to claim 7, wherein

the adjusting the intensity of the optical beam of the sensor assembly includes decreasing the intensity of the optical beam received by a receiving component of the sensor assembly.

9. The method according to claim 8, wherein

the decreasing the intensity of the received optical beam is performed by positioning a diffuser in the optical path of the optical beam between the dispensing orifice and the receiving component of the sensor assembly.

10. A method for assessing the operational flow condition for dispensing liquids through a plurality of relatively small diameter dispensing orifices aligned in a substantially linear array and each fluidly coupled to a respective communication passageway of a precision, low volume, liquid handling system, the method comprising:

(a) emitting an optical beam of a sensor assembly, from a position outboard from one side of the linear array of the dispensing orifices, along an optical path extending substantially along a longitudinal axis of the linear array, and substantially laterally across and downstream from each dispensing orifice of the liquid handling system prior to dispensing liquid from any one of the dispensing orifice;

(b) continuously sensing the optical beam along the optical path, from a position outboard from an opposite side of the linear array of the dispensing orifices;

(c) flowing the dispensing liquid through a respective communication passageway generally sufficient to eject at least a drop of dispensing liquid from one of the dispensing orifices, and across the optical path of the optical beam, wherein;

(d) detecting the drop indicates a flow condition of the dispensing fluid through the one dispensing orifice, said detecting the drop includes adjusting the sensitivity of the sensor assembly so that the respective drop of dispensed liquid from the respective dispensing orifice is more “

visible”

to the sensor assembly; and

wherein

(e) not detecting the drop indicates a non-flow condition of the dispensing fluid through the one dispensing orifice; and

(f) sequentially repeating events (c)–

(e) for the remaining dispensing orifices to assess the operational flow condition for the entire array of dispensing orifices.

11. The method according to claim 10, wherein

the emitting of an optical beam includes activating a laser diode emitting the optical beam.

12. The method according to claim 10, wherein

the continuously sensing the optical beam includes detecting the optical beam through a receiving component of said sensor assembly configured to detect the optical beam.

13. The method according to claim 10, wherein

the adjusting the sensitivity of the sensor assembly includes adjusting the sensitivity of a receiving component of said sensor assembly so that the respective drop of dispensed liquid from the respective dispensing orifice is more “

visible”

to the receiving component.

14. The method according to claim 10, wherein

the detecting the drop includes identifying a change in the continuous sensing of the optical beam, and

not detecting the drop includes not identifying a change in the continuous sensing of the optical beam.

15. The method according to claim 14, wherein

the identifying a change includes detecting the pause in the continuous sensing of the optical beam, and

the not identifying a change includes not detecting a pause in the continuous sensing of the optical beam.

16. The method according to claim 10, wherein

the adjusting the sensitivity of the sensor assembly includes adjusting the intensity of the optical beam.

17. The method according to claim 16, wherein

the adjusting the intensity of the optical beam of the sensor assembly includes decreasing the intensity of the optical beam received by a receiving component of the sensor assembly.

18. The method according to claim 17, wherein

the decreasing the intensity of the received optical beam is performed by positioning a diffuser in the optical path of the optical beam between the dispensing orifice and the receiving component of the sensor assembly.

19. A sensor assembly for assessing the liquid flow performance for dispensing liquid through a relatively small diameter dispensing orifice fluidly coupled to a communication passageway of a precision, low volume, liquid handling system, said liquid handling system being configured to flow the dispensing liquid through the communication passageway in a manner generally sufficient to eject at least a drop of dispensing liquid from the dispensing orifice, the sensor assembly comprising:

an optical emitter component positioned outboard from one side of the dispensing orifice and configured to emit an optical beam along an optical path extending substantially laterally across and downstream from the dispensing orifice of the liquid handling system prior to dispensing liquid from the dispensing orifice;

a receiving component positioned outboard from an opposite side of the dispensing orifice, and configured to continuously sense the optical beam along the optical path;

a sensitivity adjustment device cooperating between the optical emitter component and the receiving component to adjust the sensitivity of the sensor assembly so that the drop of dispensed liquid from the dispensing orifice passing across the optical path of the optical beam is more “

visible”

to the sensor assembly, wherein;

when the drop is detected, a flow condition of the dispensing fluid through the dispensing orifice is indicated; and

wherein

when the drop is not detected, a non-flow condition of the dispensing fluid through the dispensing orifice is indicated.

20. The sensor assembly according to claim 19, wherein

the optical emitter component includes a laser diode configured to emit the optical beam.

21. The sensor assembly according to claim 19, wherein

said sensitivity adjustment device is configured to adjust the sensitivity of the receiving component in a manner causing the respective drop of dispensed liquid from the respective dispensing orifice to be more “

visible”

to the receiving component.

22. The sensor assembly according to claim 21, wherein

said sensitivity adjustment device is configured to adjust the intensity of the optical beam emitted by the optical emitter component.

23. The sensor assembly according to claim 22, wherein

said sensitivity adjustment device is configured to decrease the intensity of the optical beam received by the receiving component of the sensor assembly.

24. The sensor assembly according to claim 23, wherein

said sensitivity adjustment device includes one of a diffuser and a filter positioned in the optical path of the optical beam between the dispensing orifice and the receiving component of the sensor assembly.

25. The sensor assembly according to claim 24, wherein

said adjustment device includes a cover member configured to cover the face of the receiving component, said cover member having an aperture with a diameter in the range of about 0.005 inch to about 0.030 inch.

26. The sensor assembly according to claim 25, wherein

said aperture includes a diameter in the range of about 0.020 inch.