Small volume biosensor for continuous analyte monitoring

  • US 7,045,054 B1
  • Filed: 09/19/2000
  • Issued: 05/16/2006
  • Est. Priority Date: 09/20/1999
  • Status: Expired due to Fees
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First Claim
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1. A method for monitoring analyte concentration in a biological fluid by use of a sensor, the method comprising the steps of:

  • providing a sensor comprising a volume of a hydrophilic medium for retaining an amount of analyte proportionate to the concentration of analyte in a biological fluid in contact with the sensor, an enclosure for said analyte retention volume, an electrode in contact with the hydrophilic medium, a redox enzyme in contact with the medium, and an electron transfer mediator for facilitating transfer of electrons between the enzyme and the electrode, the enzyme substantially not capable of transferring electrons to or from components of the biological fluid other than said analyte, contacting the biological fluid with the sensor and at initially predetermined intervals intermittently applying a potential to the electrode sufficient to oxidize the electron mediator and sensing current through said electrode as a function of the duration of the applied potential, maintaining the applied mediator oxidizing applied potential at least for a period of time sufficient to determine the rate of change of current with time through the electrode, and correlating the current flow with the current flow for known concentrations of said analyte in the retention medium, wherein the intervals between the intermittent applied potentials are less than the time necessary for the concentration of the analyte in the retention volume to equilibrate with that in the biological fluids in contact with the enclosure and the analyte is glucose and the redox enzyme is a glucose dehydrogenase.

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