Lentiviral LTR-deleted vector

• US 7,056,699 B2
• Filed: 06/17/2005
• Issued: 06/06/2006
• Est. Priority Date: 10/29/1996
• Status: Expired due to Fees

• Current Assignee: Oxford Biomedica (UK) Limited
• Original Assignee: Oxford Biomedica (UK) Limited
• Inventors: Kingsman, Susan Mary, Kingsman, Alan John
• Primary Examiner(s): Guzo, David
• Application Number:
  US11/155,043
• Publication Number:
  US 20050238629A1
• Time in Patent Office: 354 Days
• Field of Search: None
• US Class Current: 435/69.1
• CPC Class Codes: A61K 48/00 Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: C12N 15/62 DNA sequences coding for fusion proteins
• CPC Class Codes: C12N 15/86 Viral vectors
• CPC Class Codes: C12N 2740/15043 viral genome or elements thereof as genetic vector
• CPC Class Codes: C12N 2740/16043 viral genome or elements thereof as genetic vector
• CPC Class Codes: C12N 2799/027 where the vector is derived from a retrovirus
• CPC Class Codes: C12N 2830/00 Vector systems having a special element relevant for transcription
• CPC Class Codes: C12N 2830/15 chimeric enhancer/promoter combination
• CPC Class Codes: C12N 2830/48 regulating transport or export of RNA, e.g. RRE, PRE, WPRE, CTE
• CPC Class Codes: C12N 2830/50 regulating RNA stability, not being an intron, e.g. poly A signal
• CPC Class Codes: C12N 2830/60 from viruses
• CPC Class Codes: C12N 9/0071 acting on paired donors with incorporation of molecular oxygen (1.14)
• CPC Class Codes: C12N 9/88 Lyases (4.)

Abstract

A vector capable of transducing non-dividing and/or slowly dividing cells is provided, wherein the vector is a lentiviral LTR-deleted vector. Also provided is a method for producing a protein of interest in a non-dividing or slowly dividing cell by transducing the cell with a lentiviral LTR-deleted vector and expressing the protein of interest in the cell. In addition, target cells containing the lentiviral LTR-deleted vector are provided.

Claims

1. A lentiviral vector capable of transducing a non-dividing or slowly-dividing cell, said vector comprising a lentiviral LTR-deleted vector.

2. The lentiviral vector according to claim 1, further comprising a nucleotide sequence encoding a protein of interest.

3. A target cell in vitro comprising the lentiviral vector of claim 1.

4. A method for producing a protein of interest in a non-dividing or slowly-dividing cell, comprising the steps of:

a) transducing the cell with the lentiviral vector according to claim 2; and

b) expressing the protein of interest in the cell.

5. A target cell in vitro comprising the lentiviral vector of claim 2.

6. A method of performing gene delivery on a target cell comprising the steps of:

a) transducing the target cell with the lentiviral vector according to claim 2; and

b) delivering the nucleotide sequence to the target cell.

7. The method according to claim 4 , wherein the non-dividing cell is a neuron.

8. A lentiviral LTR-deleted vector.

9. The lentiviral LTR-deleted vector according to claim 8, further comprising a nucleotide sequence of interest.