Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays

  • US 7,083,917 B2
  • Filed: 09/26/2001
  • Issued: 08/01/2006
  • Est. Priority Date: 02/09/1996
  • Status: Expired due to Fees
First Claim
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1. A device comprising:

  • a solid support having an array of positions each suitable for attachment of an oligonucleotide probe;

    a linker suitable for coupling an oligonucleotide probe to the solid support and attached to the solid support at each of the array positions; and

    an array of capture oligonucleotide probes on the solid support at the array positions, said capture oligonucleotide probes each having greater than sixteen nucleotides and able to bind to complementary nucleic acids at uniform hybridization conditions, wherein each capture oligonucleotide probe of the array differs in sequence from its adjacent capture oligonucleotide probe, when aligned to each other by at least 25% of the nucleotides.

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