Redox polymers for use in electrochemical-based sensors

• US 7,109,271 B2
• Filed: 07/28/2004
• Issued: 09/19/2006
• Est. Priority Date: 07/28/2004
• Status: Active Grant

• Current Assignee: Cilag Gmbh International (Johnson & Johnson)
• Original Assignee: Lifescan Incorporated (Duv Holding Corporation)
• Inventors: Lillie, Geoffrey, Rodgers, James Iain, Liu, Zuifang
• Primary Examiner(s): PEZZUTO, HELEN LEE
• Application Number:
  US10/900,511
• Publication Number:
  US 20060025550A1
• Time in Patent Office: 783 Days
• Field of Search: 525/279, 525/283, 525/297, 525/298, 435/4, 435/14, 435/817
• US Class Current: 525/283
• CPC Class Codes: C12Q 1/002 Electrode membranes
• CPC Class Codes: C12Q 1/004 mediator-assisted
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

A redox polymer for use in an electrochemical-based sensor includes a hydrophobic polymer backbone (e.g., a hydrophobic poly(methyl methacrylate) polymer backbone) and at least one hydrophilic polymer arm (such as a hydrophilic oligo(N-vinylpyrrolidinone) polymer arm) attached to the hydrophobic polymer backbone. The redox polymer also includes a plurality of redox mediators (e.g., ferrocene-based redox mediators) attached to the at least one hydrophilic polymer arm.

Claims

1. A redox polymer for use in an electrochemical-based sensor, the redox polymer comprising:

a hydrophobic polymer backbone;

at least one hydrophilic polymer arm attached to the hydrophobic polymer backbone; and

a plurality of redox mediators for facilitating electron transfer between at least one redox enzyme of the electrochemical-based sensor and at least one electrode of the electrochemical-based sensor, the plurality of redox mediators attached to the at least one hydrophilic polymer arm,

wherein the hydrophobic polymer backbone is a hydrophobic poly(methyl methacrylate) polymer backbone.

2. A redox polymer for use in an electrochemical-based sensor, the redox polymer comprising:

a hydrophobic polymer backbone;

at least one hydrophilic polymer arm attached to the hydrophobic polymer backbone; and

a plurality of redox mediators for facilitating electron transfer between at least one redox enzyme of the electrochemical-based sensor and at least one electrode of the electrochemical-based sensor, the plurality of redox mediators attached to the at least one hydrophilic polymer arm,

wherein the hydrophilic polymer arm is a hydrophilic oligo(N-vinylpyrrolidinone) polymer arm.

3. A redox polymer for use in an electrochemical-based sensor, the redox polymer comprising:

a hydrophobic polymer backbone formed from methyl methacrylate (MMA) monomers;

at least one hydrophilic polymer arm attached to the hydrophobic polymer backbone and formed from N-vinylpyrrolidinone (N/P) monomers; and

a plurality of ferrocene redox mediators attached to the at least one hydrophilic arm and formed from vinyl ferrocene (VFc).

4. The redox polymer of claim 3, wherein the mole ratio of MMA monomers to hydrophilic polymer arms is in the range of about 50:

1 to 150;

1.

5. The redox polymer of claim 4, wherein the mole ratio of NVP to VFc is in the range of about 100:

1 to about 100;

5.

6. The redox polymer of claim 5, wherein the redox polymer has a molecular weight in the range of 20 Kg/mole to 80 Kg/mole.