Label-free high-throughput optical technique for detecting biomolecular interactions

• US 7,118,710 B2
• Filed: 08/15/2001
• Issued: 10/10/2006
• Est. Priority Date: 10/30/2000
• Status: Expired due to Fees

• Current Assignee: X-Body Incorporated (Bristol-Myers Squibb Company), CoHo Holdings LLC
• Original Assignee: Sru Biosystems Incorporated
• Inventors: Cunningham, Brian
• Primary Examiner(s): Snay, Jeffrey R.
• Application Number:
  US09/929,957
• Publication Number:
  US 20020168295A1
• Time in Patent Office: 1,882 Days
• Field of Search: 422/82.05, 422/82.09, 422/82.11, 436/518, 436/524, 436/525, 436/527, 436/164, 436/165, 385/37, 385/12, 385129-132, 356/128, 359/572
• US Class Current: 422/82.09
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/4788 Diffraction for sizing particles G01N15/0205
• CPC Class Codes: G01N 21/7743 the reagent-coated grating coupling light in or out of the waveguide
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G02B 5/1809 with pitch less than or comparable to the wavelength
• CPC Class Codes: Y10T 436/24 Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry

Abstract

Methods and compositions are provided for detecting biomolecular interactions. The use of labels is not required and the methods can be performed in a high-throughput manner. The invention also provides optical devices useful as narrow band filters.

Claims

1. A biosensor comprising:

(a) a two-dimensional grating comprised of a material having a high refractive index, wherein the two-dimensional grating is comprised of a repeating pattern of lines;

(b) a substrate layer that supports the two-dimensional grating; and

(c) one or more specific binding substances immobilized on the surface of the two-dimensional grating opposite of the substrate layer;

wherein, when the biosensor is illuminated a resonant grating effect is produced on the reflected radiation spectrum, and wherein the depth and period of the two-dimensional grating are less than the wavelength of the resonant grating effect.

2. A detection system comprising the biosensor of claim 1, a light source that directs light to the biosensor, and a detector that detects light reflected from the biosensor.

3. The detection system of claim 2, further comprising a fiber probe comprising one or more illuminating optical fibers that are connected at a first end to the light source, and one or more collecting optical fibers connected at a first end to the detector, wherein the second ends of the illuminating and collecting fibers are arranged in line with a collimating lens that focuses light onto the biosensor.

4. The detection system of claim 3, wherein the illuminating fiber and the collecting fiber are the same fiber.

5. The detection system of claim 2, wherein the light source illuminates the biosensor from its top surface or from its bottom surface.