Automated process for preparing and amplifying a target nucleic acid sequence

  • US 7,118,892 B2
  • Filed: 10/03/2002
  • Issued: 10/10/2006
  • Est. Priority Date: 05/01/1998
  • Status: Expired due to Term
First Claim
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1. A process for preparing and amplifying a target sequence contained within a target nucleic acid present in a fluid sample, the process comprising performing in a stand-alone unit the ordered and automated steps of:

  • a) immobilizing the target nucleic acid on a solid support;

    b) separating other material present in the fluid sample from the target nucleic acid immobilized in step a);

    c) washing the solid support one or more times with a wash solution; and

    d) amplifying the target sequence in a receptacle containing the target nucleic acid and amplification reagents provided thereto, the receptacle being formed to have an open top end and a closed bottom end wherein the stand-alone unit comprises first and second stations, and wherein steps a)–

    c) are performed at the first station and step d) is performed at the second station.

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