Polymerase chain reaction assays for monitoring antiviral therapy and making therapeutic decisions in the treatment of acquired immunodeficiency syndrome

  • US 7,129,041 B2
  • Filed: 12/16/2002
  • Issued: 10/31/2006
  • Est. Priority Date: 05/14/1992
  • Status: Expired due to Fees
First Claim
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1. A method of evaluating the effectiveness of anti-HIV therapy of a patient comprising:

  • correlating the presence or absence of detectable HIV-encoding nucleic acid in a plasma sample of an HIV infected patient with an absolute CD4 count, wherein the presence or absence of said detectable HIV-encoding nucleic acid is determined by(i) collecting a plasma samples from an HIV-infected patient who is being treated with an antiretroviral agent;

    (ii) amplifying HIV-encoding nucleic acid that may be present in the plasma sample using HIV primers via PCR and;

    (iii) testing for the presence of HIV-encoding nucleic acid sequence in the product of the PCR.

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