Data frame selection for cytometer analysis
First Claim
Patent Images
1. A method for detecting an optimum core stream in a channel, comprising:
- at a starting time, providing a sample of particles to form a core stream that flows in a channel;
directing light at the particles of the core stream flowing in the channel;
detecting light scattered by the particles in the core stream during a series of time intervals;
converting the light scattered by the particles detected at a forward angle light scattering (FALS) angle and a small angle light scattering (SALS) angle, into FALS versus SALS data for each interval of time of the series of time intervals after the starting time;
analyzing the FALS versus SALS data for each interval of time; and
selecting a time duration from the series of time intervals where the FALS versus SALS data exhibit an approximately certain count and grouping of data.
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Abstract
A system for detecting when a core stream in a channel of a flow device is adequate for sufficient data accession. The system may determine the time period from the beginning of a sample run in a channel of a flow device to when the core stream is adequate. Data of detected FALS versus SALS light scattered by particles of the core stream may be plotted in data frames during a series of time intervals. The quality of the grouping and count rate of the data may indicate which frame and corresponding time interval reveal an adequate core stream.
114 Citations
42 Claims
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1. A method for detecting an optimum core stream in a channel, comprising:
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at a starting time, providing a sample of particles to form a core stream that flows in a channel; directing light at the particles of the core stream flowing in the channel; detecting light scattered by the particles in the core stream during a series of time intervals; converting the light scattered by the particles detected at a forward angle light scattering (FALS) angle and a small angle light scattering (SALS) angle, into FALS versus SALS data for each interval of time of the series of time intervals after the starting time; analyzing the FALS versus SALS data for each interval of time; and selecting a time duration from the series of time intervals where the FALS versus SALS data exhibit an approximately certain count and grouping of data. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. An optimization detection system comprising:
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a processor connected to a FALS and SALS detector mechanism situated proximate to a light source arrangement having a flow channel situated between the FALS and SALS detector mechanism and the light source arrangement; and wherein; the processor is for recording signals from the FALS and SALS detector mechanism; the processor is further for providing an output comprising a FALS signal versus a SALS signal for each particle detected in the flow channel; the output comprises a plurality of frames; each frame of the plurality of frames contains a plot of the FALS signal versus the SALS signal for each particle detected in the flow channel for a time interval; and the time interval is of a series of time intervals beginning at an entry of particles into the flow channel to form a core stream. - View Dependent Claims (16, 17, 18)
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19. A method for detecting an optimum core stream in a flow channel, comprising:
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beginning an insertion of particles to form a core stream sheathed by a fluid to flow in a channel; directing light at the particles in the channel; detecting and converting light scattered by the particles into electrical signals; processing the electrical signals in accordance with an algorithm for a determination of when the core stream is optimum for data taking; measuring the lapsed time between the insertion of particles to form the core stream and when the core steam is optimum for data taking; and plotting the electrical signals as FALS versus SALS data in a frame during a time interval; and wherein; the detecting light scattered by the particles is of light at a forward angle light scattering (FALS) angle and a small angle light scattering (SALS) angle; a series of time intervals occur during the lapsed time after the insertion of particles; and the frames of the time intervals are evaluated and an at least one frame is selected indicating that the core stream is optimum for data taking. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26, 27, 28, 29)
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30. Means for detecting an optimum core stream in a channel of a flow device, comprising:
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means for providing particles to form a core stream that flows in a channel starting at time zero; means for directing light at the particles of the core stream flowing in the channel; and means for determining an optimum core stream from light scattered by the particles; and wherein the means for determining an optimum core stream comprises; means for detecting light scattered by the particles at a set of scattering angles; means for converting detected light into data signals; means for analyzing the data signals for each time interval after time zero; and means for selecting a time interval where the data signals indicate an optimum core stream sufficient for data accession. - View Dependent Claims (31, 32, 33, 34, 35)
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36. A method for detecting an optimum core stream in a channel, comprising:
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providing a sample of particles to form a core stream that flows in a channel; directing light at the particles of the core stream flowing in the channel; detecting light scattered by the particles in the core stream during a series of time intervals; converting the light, scattered by the particles detected at a first light scattering angle and a second light scattering angle, into first light scattering angle versus second light scattering angle data for a series of time intervals; analyzing the first light scattering angle versus second light scattering angle data for the series of time intervals; and selecting a time duration from the series of time intervals where the first light scattering angle versus second light scattering angle data exhibit an approximately certain count and grouping of data. - View Dependent Claims (37, 38, 39, 40, 41, 42)
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Specification