Method for detecting PCR product using electrical signal

• US 7,135,294 B2
• Filed: 09/09/2003
• Issued: 11/14/2006
• Est. Priority Date: 11/12/2002
• Status: Expired due to Fees

• Current Assignee: Samsung Electronics Co. Ltd.
• Original Assignee: Samsung Electronics Co. Ltd.
• Inventors: Lee, Jae-hoon, Yoon, Dae-sung, Lim, Geun-bae, Lee, Jae-chang
• Primary Examiner(s): Nguyen, Vincent Q.
• Assistant Examiner(s): NATALINI, JEFF WILLIAM
• Application Number:
  US10/658,169
• Publication Number:
  US 20040100284A1
• Time in Patent Office: 1,162 Days
• Field of Search: 324/558, 324/660, 324/661, 324/663, 205/775, 205/777.5, 204/403.01, 204/403.02, 204/194, 422/91.1, 422/91.2, 435/91.2, 435/6
• US Class Current: 435/6.11
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors
• CPC Class Codes: C12Q 1/686 Polymerase chain reaction [PCR]
• CPC Class Codes: C12Q 2531/113 PCR
• CPC Class Codes: C12Q 2561/113 Real time assay
• CPC Class Codes: C12Q 2565/607 being a sensor, e.g. electrode
• CPC Class Codes: G01N 27/221 by investigating the dielectric properties using microwaves G01N22/00; measuring loss factors or dielectric constants per se G01R27/26

Abstract

A method for detecting a polymerase chain reaction (PCR) product is provided. The method includes (a) providing at least a pair of electrodes in a PCR solution-containing vessel; (b) performing PCR; (c) producing an electric field between the electrodes; and (d) measuring a change in a dielectric property in the PCR solution. Therefore, a PCR product can be detected in real time.

Claims

1. A method for detecting a polymerase chain reaction (PCR) product, comprising

providing at least a pair of electrodes in a PCR solution-containing vessel, wherein the pair of electrodes is connected to an impedance sensor;

performing PCR;

producing an electric field between the pair of electrodes; and

measuring a change in impedance magnitude of the PCR solution,

wherein the measuring is performed in the absence of an additional probe for generating an electrical signal and at a predetermined alternating current voltage frequency with an increase in the number of PCR cycles.

2. The method according to claim 1, wherein the predetermined alternating current voltage frequency is about 1,000 Hz.