Microorganism culture medium

• US 7,141,387 B2
• Filed: 07/12/2005
• Issued: 11/28/2006
• Est. Priority Date: 07/12/2004
• Status: Active Grant

• Current Assignee: JNC Corporation (Chisso Corporation)
• Original Assignee: Chisso Corporation
• Inventors: Ushiyama, Masashi
• Primary Examiner(s): Lilling, Herbert J.
• Application Number:
  US11/179,098
• Publication Number:
  US 20060008867A1
• Time in Patent Office: 504 Days
• Field of Search: 435/7.2, 435/29, 435/34, 435/253.6, 435/255.7
• US Class Current: 435/34
• CPC Class Codes: C12N 1/20 Bacteria; Culture media therefor
• CPC Class Codes: C12Q 1/04 Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor C12Q1/6897 takes precedence
• CPC Class Codes: C12Q 1/045 Culture media therefor

Abstract

A microorganism culture medium comprising a derivative A′ of a chromogen A, and a chromogen B, where the chromogen A is a chromogen being oxidized to a colorant, and the chromogen B is a chromogen being reduced to a colorant. The derivative A′ acts as a substrate of an enzyme generated when a microorganism is growing, and is then converted to the chromogen A, and the derivative A′ and the chromogen B are substantially not colored in the culture medium unless a microorganism is inoculated.

Claims

1. A microorganism culture medium comprising a derivative A′

of a chromogen A, and a chromogen B,

wherein the chromogen A is a chromogen being oxidized to a colorant, and the chromogen B is a chromogen being reduced to a colorant,

the derivative A′

acts as a substrate of an enzyme generated when a microorganism is growing, and is then converted to the chromogen A,

and the derivative A′ and

the chromogen B are substantially not colored in the culture medium unless a microorganism is inoculated.

2. The microorganism culture medium of claim 1, wherein the derivative A′

of the chromogen A is an oxyindole derivative or a halogen-substituted oxyindole derivative.

3. The microorganism culture medium of claim 1, wherein the chromogen B is a tetrazolium salt having a half wave potential of 300 mv or less as an absolute value.

4. The microorganism culture medium of claim 2, wherein the oxyindole derivative or the halogen-substituted oxyindole derivative is an acetic acid ester, a fatty acid ester, a phosphoric acid ester, a sulfuric acid ester or a glucoside.

5. The microorganism culture medium described in the claim 1, wherein the medium is a sheet type medium.

6. The microorganism culture medium described in claim 1, wherein the medium is an agar medium.

7. A method for detecting microorganisms using the medium described in claim 1.